In vitro antioxidant and antidiabetic potential of herbal mixture traditionally used in treatment of diabetes mellitus

Aleksandra Petrović, Katarina Stojković, Višnja Madić, Ljubiša Đorđević


This work aimed to assess and compare the phytochemical composition, the free radical neutralization capacity, and antidiabetic potential of a traditional herbal mixture and its individual ingredients: Apium graveolens, Polygonum aviculare, Hypericum perforatum, and Conyza canadensis. Antioxidative capacity was evaluated using DPPH radical scavenging test. Antidiabetic activity was assessed using α-amylase inhibition assay. The results showed that there was a positive correlation between antioxidant and antidiabetic activity with total phenol (p < 0.001) and flavonoid content (p < 0.001) in all tested extracts. Herbal mixture had higher total phenol and flavonoid content than most of its individual ingredients. Its antioxidant capacity was excellent, even higher than standard, butylated hydroxytoluene (p < 0.001). Moreover, it showed significant antidiabetic potential (IC50 value 99.70±8.4 μg/ml). To conclude, the herbal mixture showed great potential for the future use as a dietary supplement in the therapy for diabetes.


herbal mixture, antioxidative activity, antidiabetic activity, DPPH test, α-amylase inhibition assay

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