Antimicrobial efficacy of basil and sage essential oils: time-lapse kinetics and type of interaction with ciprofloxacin

Milica Pejčić, Zorica Stojanović- Radić, Marina Dimitrijević, Niko Radulović

Abstract


The increasing resistance of Pseudomonas aeruginosa strains to used antibiotics is a big problem of modern medicine. Finding antimicrobial substances which will increase the antibiotics effects is necessary. Therefore, the aim of the present study was to evaluate the time-lapse activity of the basil and sage oils, to evaluate the combined oils effect against P. aeruginosa clinical isolates and to determine the oils potential to enhance the activity of ciprofloxacin.

The obtained growth curves showed a reduction in the number of bacterial cells in the range of 10.5-94% when basil oil was applied. In the case of the sage oil, higher reduction has been observed (48.5-100%). The basil oil achieved a synergistic interaction with ciprofloxacin in 8 isolates, while the same effect for sage/ciprofloxacin combination was observed in 6 isolates. Indifferent effect was noticed in 64% of the tested isolates for basil/sage combination. These data showed potential benefits of the oils and ciprofloxacin combination therapy in the treatment of P. aeruginosa infections.


Keywords


Basil oil; sage oil; time-lapse activity; synergy; ciprofloxacin; Pseudomonas aeruginosa

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