Effects of different sucrose concentrations on some parameters of the life cycle in two wild Drosophila species

Vladimir Cvetković, Saša Stanković, Vladimir Žikić, Nikola Jovanović, Jovana Dimitrijević, Aleksandra Cvetanović, Tatjana Mitrović

Abstract


The fruit flies collected from the wild can be easily cultured in the laboratory for research purposes. On that occasion, many similar recipes for cornmeal-based feeding media can be used for Drosophila cultivation. However, in these recipes concentrations of ingredients may differ what might imply a need to choose the recipe which is the most appropriate for culturing of wild Drosophila species. The aim of this study was to check how two wild species, Drosophila melanogaster and Drosophila suzukii respond to different sucrose concentration in feeding media by monitoring of life cycle key parameters. The total number of oviposited eggs, formed pupae and eclosed adults, as well as dynamics of pupae formation and adult eclosion, were recorded. The results showed that the sucrose concentrations of 160 g×L-1 caused a significantly lower number of formed pupae and eclosed adults in D. suzukii than at concentrations of 40 g×L-1 and 80 g×L-1. Prolonged and stretched dynamics of pupation and eclosion were recorded in D. melanogaster only at a concentration of 160 g×L-1. All tested Drosophila showed no differences in life cycle parameters between sucrose concentration of 40 g×L-1 and 80 g×L-1. Presented results can be helpful in deciding which cornmeal-based feeding media recipe to choose for the cultivation of mentioned wild Drosophila species.


Keywords


Fruit fly; Drosophila melanogaster; Drosophila suzukii; feeding media; life cycle; sucrose concentration

Full Text:

PDF

References


Anagnostou, C., Dorsch, M., Rohlfs, M. 2010: Influence of dietary yeasts on Drosophila melanogaster life‐history traits. Entomologia Experimentalis et Applicata, 136(1): 1-11.

Bellutti, N., Gallmetzer, A., Innerebner, G., Schmidt, S., Zelger, R., Koschier, E.H. 2018: Dietary yeast affects preference and performance in Drosophila suzukii. Journal of pest science, 91: 651-660.

Benito, N.P., Lopes-da-Silva, M., Santos, R.S. 2016: Potential spread and economic impact of invasive Drosophila suzukii in Brazil. Pesquisa Agropecuária Brasileira, 51(5):571-578.

Cvetković, V.J., Jovanović, B., Lazarević, M., Jovanović, N., Savić-Zdravković, D., Mitrović, T., Žikić, V. 2020: Changes in the wing shape and size in Drosophila melanogaster treated with food grade titanium dioxide nanoparticles (E171) – A multigenerational study. Chemosphere. Dec 1;261: 127787.

Cvetković, V. J., Mitrović, T. L., Jovanović, B., Stamenković, S. S., Todorović, M., Đorđević, M., Radulović, N. 2015: Toxicity of dimethyl sulfoxide against Drosophila melanogaster. Biologica Nyssana, 6(2): 91-95.

De Ros, G., Anfora, G., Grassi, A., Ioriatti, C. 2013: The potential economic impact of Drosophila suzukii on small fruits production in Trentino (Italy). IOBC-WPRS Bulletin, 91:317-321.

Fellous, S. & Xuéreb, A. 2017: A geometric analysis of the macronutrient needs of Drosophila suzukii larvae. Drosophila Information Service, 100: 158-167.

Gao, H.H., Zhai, Y.F., Chen, H., Wang, Y.M., Liu, Q., Hu, Q.L., Ren, F.S., Yu, Y. 2018: Ecological niche difference associated with varied ethanol tolerance between Drosophila suzukii and Drosophila melanogaster (Diptera: Drosophilidae). Florida Entomologist, 101(3): 498-504.

Jovanović, B., Cvetković, V.J., Mitrović, T.L. 2016: Effects of human food grade titanium dioxide nanoparticle dietary exposure on Drosophila melanogaster survival, fecundity, pupation and expression of antioxidant genes. Chemosphere, 144: 43-49.

Jovanović, B., Jovanović, N., Cvetković, V.J., Matić, S., Stanić, S., Whitley, E.M., Mitrović, T.L. 2018: The effects of a human food additive, titanium dioxide nanoparticles E171, on Drosophila melanogaster-a 20 generation dietary exposure experiment. Scientific reports, 8: 17922.

Kinjo, H., Kunimi, Y., Nakai, M. 2014: Effects of temperature on the reproduction and development of Drosophila suzukii (Diptera: Drosophilidae). Applied entomology and zoology, 49: 297-304.

Lee, K.P. 2015: Dietary protein: carbohydrate balance is a critical modulator of lifespan and reproduction in Drosophila melanogaster: a test using a chemically defined diet. Journal of insect physiology, 75:12-19.

Lewis, E.B. 1960: A new standard food medium. Drosophila Information Service, 34: 117–118.

Lin, Q.C., Zhai, Y.F., Zhang, A.S., Men, X.Y., Zhang, X.Y., Zalom, F.G., Zhou, C.G., Yu, Y. 2014: Comparative developmental times and laboratory life tables for Drosophlia suzukii and Drosophila melanogaster (Diptera: Drosophilidae). Florida Entomologist, 97(4): 1434-1442.

Lushchak, O.V., Gospodaryov, D.V., Rovenko, B.M., Yurkevych, I.S., Perkhulyn, N.V., Lushchak, V.I. 2014: Specific dietary carbohydrates differentially influence the life span and fecundity of Drosophila melanogaster. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, 69(1):3-12.

Mazzi, D., Bravin, E., Meraner, M., Finger, R., Kuske, S. 2017: Economic impact of the introduction and establishment of Drosophila suzukii on sweet cherry production in Switzerland. Insects, 8(1):18.

McKenzie, J.A., Parsons, P.A. 1972: Alcohol tolerance: an ecological parameter in the relative success of Drosophila melanogaster and Drosophila simulans. Oecologia, 10: 373–388.

Schlesener, D.C., Wollmann, J., Krüger, A.P., Martins, L.N., Geisler, F.C., Garcia, F.R. 2017: Rearing method for Drosophila suzukii and Zaprionus indianus (Diptera: Drosophilidae) on artificial culture media. Drosophila Information Service, 100: 185-189.

Silva-Soares, N.F., Nogueira-Alves, A., Beldade, P., Mirth, C.K. 2017: Adaptation to new nutritional environments: larval performance, foraging decisions, and adult oviposition choices in Drosophila suzukii. BMC ecology, 17: 21.

Tochen, S., Dalton, D.T., Wiman, N., Hamm, C., Shearer, P.W., Walton, V.M. 2014: Temperature-related development and population parameters for Drosophila suzukii (Diptera: Drosophilidae) on cherry and blueberry. Environmental entomology, 43(2): 501-510.

Toševski, I., Milenković, S., Krstić, O., Kosovac, A., Jakovljević, M., Mitrović, M., Cvrković, T., Jović, J. 2014: Drosophila suzukii (Matsumura, 1931)(Diptera: Drosophilidae): A new invasive pest in Serbia. Zaštita bilja, 65(3): 99-104.

https://bdsc.indiana.edu


Refbacks

  • There are currently no refbacks.