Effects of drought stress factors on antibacterial activity of two Triticum aestivum L. varieties

Okan Acar, Nurcihan Hacıoğlu Doğru


Triticum aestivum L. (Wheat grass), one of the members of Poaceae family, has been considered for very efficient therapeutic drugs. Current study was aimed at evaluation of antibacterial properties of two varieties of T. aestivum L. [cv. Tosunbey (drought tolerant) and cv. Sultan 95 (drought sensitive)] which grown in three different stress condition [(1) drought stress; (2) pre-treatment of seeds with acetyl salicylic acid; (3) drought stress and pre-treatment of seeds with acetyl salicylic acid]. The antibacterial activity of the ethanol extracts were assayed against five pathogens (Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Escherichia coli NRRL B-3704, Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633) by agar disc diffusion method and micro broth dilution methods. The results of showed that the ethanol extracts from the different treatments studied showed antibacterial  activities, with the diameters of the inhibition zone ranging from 8 to 15 mm and 2.5 to 20 µg/mL, respectively. But, the highest antibacterial activity against B. subtilis ATCC 6633 were demonstrated by the extract of T. aestivum cv. Sultan 95 which grown in drought stress and pre-treatment of seeds with acetyl salicylic acid.

Full Text:



Ashok, S.A. 2011: Phytochemical and pharmacological screening of wheat grass Juice (Triticum aestivum L.). International Journal of Pharmaceutical Sciences Review and Research, 9: 159-164.

Bhattacharjee, B., Islam, S.M.S. 2015: Assessment of antibacterial and antifungal activities of the extracts of Rhynchostylis retusa. Blume- A Medicinal Orchid. World Journal of Pharmacy and Pharmaceutical Sciences, 4: 74-87.

Das, K., Tiwari, R.K.S., Shrivastava, D.K. 2010: Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research, 4: 104-111.

Das, A,, Raychaudhuri, U,, Chakraborty, R. 2012: Antimicrobial effect of edible plant extract on the growth of some foodborne bacteria including pathogens. Nutrafoods, 12: 83-88.

Duke, J.A., Ayensu, E.S. 1985: Medicinal Plants of the World. Algonac, MI (USA): Reference Publications.

Ejike, C.E.C.C., Gong, M., Udenigwe, C.C. 2013: Phytoalexins from the Poaceae: Biosynthesis, function and prospects in food preservation. Food Research International, 52 (1), 167-177.

Hadjivassiliou, M., Grunewald, R.A., Sharrack, B., Sanders, D., Lobo, A., Williamson. C. Woodroofe, N., Wood, N., Davies-Jones, A. 2003: Gluten ataxia in perspective: epidemiology, genetic susceptibility and clinical characteristics. Brain, 126: 685-691.

Hossein, S., Hosseini, S.M., Azari, A., Rafsanjani, M. H. 2018: Effects of seed priming with ABA and SA on seed germination and seedling growth of sesame (Sesamum indicum L.) under saline condition. Australian J of Crop Sci., 12 (9), 1385-1392.

Li, H., Goodwin P.H., Han, Q., Huang, L., Kang, Z. 2011: Microscopy and proteomic analysis of the non-host resistance of Oryza sativa to the wheat leaf rust fungus, Puccinia triticina f. sp. tritici. Plant Cell Reports, 31 (4): 637-650.

Padalia, S., Drabu, S., Raheja, I., Gupta, A., Dhamija, M. 2010: Multitude potential of wheatgrass juice (Green Blood): An overview. Chronicles of Young Scientists, 1(2): 23-28.

Pallavi, K., Kumarswammy, G., Shruthi. B. 2011: Pharmacognostic investigation and antibacterial activity of Triticum aestivum. Journal of Pharmacy Research, 4: 3355-3359.

Rojas, A., Hernandez, L., Pereda-Miranda, R., Mata, R. 1992: Screening for antimicrobial activity of crude drug extracts and pure natural products from Mexican medicinal plants. Journal of Ethnopharmacology, 35: 275-83.

Safi, H., Saibi, W., Alaoui, M.M., Hmyene, A., Masmoudi, K., Hanin, M., Brini, F. 2015: A wheat lipid transfer protein (TdLTP4) promotes tolerance to abiotic and biotic stress in Arabidopsis thaliana. Plant Physiology and Biochemistry, 89, 64-75.

Saha, S., Islam, Z., Islam, S., Hossain, S., Islam, S.M.S. 2018: Evaluation of antimicrobial activity of wheat (Triticum aestivum L.) against four bacterial strains. SKUAST Journal of Research, 20 (1): 58-62.

Schmidt, M., Arendt, E.K., Thery, T.L.C. 2019: Isolation and characterisation of the antifungal activity of the cowpea defensin Cp-thionin II. Food Microbiology, 82: 504–514.

Sehgal, O.P., Mohamad, F. 2018: Pathogenesis related proteins. In: Mandahar, C.L. (Ed.) Plant Viruses Vol. II: Pathology: 65-84. CRC Press, Boca Raton.

Sharma, A., Sharma, D., Verma, S.K. 2018: In silico study of iron, zinc and copper binding proteins of Pseudomonas syringae pv. lapsa: Emphasis on secreted metalloproteins. Frontiers in Microbiology, 9: 1838.

Steward F.C. 1983: Plant Physiology, Academic Press, New York, 797p.

Sundaresan, A., Selvi, A., Manonmani, H.K. 2015: The anti-microbial properties of Triticum aestivum (Wheat Grass). International Journal of Biotechnology for Wellness Industries, 4: (3): 84-91.

Van Loon, L.C., Rep, M.P. 2006: Significance of inducible defense-related proteins in infected plants. Annual Review of Plant Pathology, 44: 135-162.

Gregorova, Z., Kovacik, J., Klejdus, B., Maglovski, M., Kuna, R., Hauptvogel, P., Matusíkova, I. 2015: Drought-Induced Responses of Physiology, Metabolites, and PR Proteins in Triticum aestivum. J of Agric and Food Chem., 63(37): 8125-8133.

Thiruvengadam, M., Baskar, V., Kim, S.-H., Chung, I.M. 2016: Effects of abscisic acid, jasmonic acid and salicylic acid on the content of phytochemicals and their gene expression profiles and biological activity in turnip (Brassica rapa ssp. rapa). Plant Growth Regulation, 80 (3): 377-390.

Westh, H., Zinn, C.S., Rosdahl V.T. 2004: An international multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries. Microbial Drug Resistance, 10: 169-76.


  • There are currently no refbacks.