Autecology and ex situ growth of Onobrychis pindicola Hausskn. subsp. urumovii Deg. & Dren. (Fabaceae) – endemic with medicinal potential.

Ekaterina Kozuharova, Robert Nash


 Abstract: Onobrychis pindicola subsp. urumovii Degen & Dren. is an endemic with very restricted distribution on just two mountains Pirin Mts. and Slavjanka Mts. SW Bulgaria. The taxon is evaluated as least concerned by the IUCN criteria but it is an element in several Natura 2000 habitats with conservation significance. The aim of this study is to investigate the microhabitat specifics of O. pindicola subsp. urumovii, namely slope, exposure, bed rock, soils, and vegetation as well as spatial distribution and phenology regarding the possible future cultivation. Basically O. pindicola subsp. urumovii demonstrates high tolerance to its environment although it is a calciphilous species. It grows successfully ex situ in experimental plot in the foothills of the mountains, which indicates prospects for successful cultivation. This will be important in case of future industrial necessity of the plant substance for medicinal purposes. 


ex situ, microhabitat, Onobrychis pindicola subsp. urumovii, phosphorus, soil pH

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Agelet, A., Valles, J. 2001: Studies on pharmaceutical ethnobotany in the region of Pallars (Pyrenees, Catalonia, Iberian Peninsula). Part I. General results and new or very rare medicinal plants. Journal of Ethnopharmacology 77(1): 57-70.

Anonymous, 2004: Pirin National Park Management Plan NP Direction, Bansko,

Anonymous, 2009: Pirin WHC Nomination 2009 - Pirin National Park prpoposed as world heritage property 2009

Blériot, Y., Gretzke, D., Krülle, T. M., Butters, T. D., Dwek, R. A., Nash, R. J., Asano, N., Fleet, G. W. 2005: Looking glass inhibitors: efficient synthesis and biological evaluation of d-deoxyfuconojirimycin. Carbohydrate research, 340(18): 2713-2718.

Bonet, M. À., Parada, M., Selga, A., Valles, J. 1999: Studies on pharmaceutical ethnobotany in the regions of L’Alt Emporda and Les Guilleries (Catalonia, Iberian Peninsula). Journal of Ethnopharmacology 68(1): 145-168.

Dullinger, S., Dirnböck, T., Grabherr, G. 2000: Reconsidering endemism in the North-eastern Limestone Alps. Acta Botanica Croatica 59(1): 55-82. URI

Euro+Med PlantBase 2011: accessed 4 February 2016, 2005 Dipartimento di Scienzeambientali e Biodiversità ed Orto botanico, Università degli Studi di Palermo, Page editor: G. Domina. These pages last updated 10 March, 2011.

Ghasemi, P. A., Momeni, M., Bahmani, M. 2013: Ethnobotanical study of medicinal plants used by Kurd tribe in Dehloran and Abdanan districts, Ilam province, Iran. African Journal of Traditional, Complementary and Alternative Medicines 10(2): 368-385.

Gutterson, N., Ralston, E. 2002: U.S. Patent No. 6,392,119. Washington, DC: U.S. Patent and Trademark Office.

Halabalaki, M., Alexi, X., Aligiannis, N., Alexis, M. N., Skaltsounis, A. L. 2008: Ebenfurans IV− VIII from Onobrychis ebenoides: Evidence that C-Prenylation is the Key Determinant of the Cytotoxicity of 3-Formyl-2-arylbenzofurans. Journal of Natural Products 71(11): 1934-1937. DOI: 10.1021/np800134h

Halabalaki, M., Alexi, X., Aligiannis, N., Kalpoutzakis, E., Alexis, M. N., Skaltsounis, A.L. 2008: Onobrychis species: a source of novel C-prenylated cytotoxic 3-formyl-2-arylbenzofurans. Planta Medica 74(9): 1079-1079. DOI: 10.1055/s-0028-1084505

Halabalaki, M., Alexi, X., Aligiannis, N., Lambrinidis, G., Pratsinis, H., Florentin, I., Alexis, M. N. 2006: Estrogenic activity of isoflavonoids from Onobrychis ebenoides. Planta medica 72(6): 488.

Halabalaki, M., Aligiannis, N., Papoutsi, Z., Mitakou, S., Moutsatsou, P., Sekeris, C., Skaltsounis AL (2000) Three new arylobenzofurans from onobrychis ebenoides and evaluation of their binding affinity for the estrogen receptor. Journal of Natural Products 63(12): 1672-1674.

Hegland, S. J., Totland, Ø. 2012: Interactions for pollinator visitation and their consequences for reproduction in a plant community. Acta Oecologica 43, 95-103.

Heinrich, B. 1979: " Majoring" and" minoring" by foraging bumblebees, Bombus vagans: an experimental analysis. Ecology, 60(2): 245-255.

Heinrich, B. 1981: The energetics of pollination. Annals of the Missouri Botanical Garden 68(2): 370-378. DOI: 10.2307/1937652

Ince, S., Fi̇lazi̇, A. 2009: Determined of phytochemical properties of sainfoin (Onobrychis viciifolia) and acute orally LD50 in mice. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56(4): 263-267.

Jones, G. A., McAllister, T. A., Muir, A. D., Cheng, K. J. 1994: Effects of sainfoin (Onobrychis viciifolia Scop.) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Applied and Environmental Microbiology 60(4): 1374-1378.

Jordanov, D. (ed) 1963-2012: Fl. Reipubl. Popularis Bulgaricae. Vol. 1-11, In Aedibus Acad. Sci. Bulgaricae, Serdicae

Joudi, L., Bibalani, G. H. 2010: Exploration of medicinal species of Fabaceae, Lamiaceae and Asteraceae families in Ilkhji region, Eastern Azerbaijan Province (Northwestern Iran). Journal of Medicinal Plants Research 4(11): 1081-1084.

Kato, A., Zhang, Z. L., Wang, H. Y., Jia, Y. M., Yu, C. Y., Kinami, K., Hirokami, Y Tsuji, I Adachi, RJ, Nash, Fleet, G. W. 2015: Design and Synthesis of Labystegines, Hybrid Iminosugars from LAB and Calystegine, as Inhibitors of Intestinal α-Glucosidases: Binding Conformation and Interaction for ntSI. The Journal of Organic Chemistry, 80(9): 4501-4515. DOI: 10.1021/acs.joc.5b00342

Katsanou, E. S., Halabalaki, M., Aligiannis, N., Mitakou, S., Skaltsounis, A. L., Alexi, X., Alexis, M. N. 2007: Cytotoxic effects of 2-arylbenzofuran phytoestrogens on human cancer cells: modulation by adrenal and gonadal steroids. The Journal of Steroid Biochemistry and Molecular Biology 104(3): 228-236.

Kozuharov, S. (1976) Onobrychis Adans. —In: Jordanov, D. (ed.): Flora Republicae Popularis Bulgaricae. Sofia, 236-258 (in Bulgarian)

Kozuharova, E. 1999: On the reproductive biology of Onobrychis pindicola Hausskn. subsp. urumovii Deg. & Dren.(F abaceae). Flora Mediterranea. 9, 291-303.

Kozuharova, E., Raimondo, F. M. 2003: Plant communities growing on marble slopes or North Pirin Mts (SW Bulgaria)-blossom morphology and bee visitors or a complex midsummer flowering entomophylus plants. Bocconea 16(2): 935-951.

Kozuharova, E., Richards, A., Ninov, N., Kostov, K., Aleksandrov, B. 2012: Autecological observations on Oxytropis species (Fabaceae)–two of them rare and endemic from Northern Pirin Mts, Bulgaria. Phytologia Balcanica 18(1): 25-35.

Łuczaj. Ł. 2012: Ethnobotanical review of wild edible plants of Slovakia. Acta Societatis Botanicorum Poloniae, 81(4): 245-255.

Mercer, T. B., Jenkinson, S. F., Bartholomew, B., Nash, R. J., Miyauchi, S., Kato, A., Fleet, G. W. 2009: Looking glass inhibitors: both enantiomeric N-benzyl derivatives of 1, 4-dideoxy-1, 4-imino-D-lyxitol [a potent competitive inhibitor of α-D-galactosidase] and of 1, 4-dideoxy-1, 4-imino-L-lyxitol [a weak competitive inhibitor of α-D-galactosidase] inhibit naringinase, an α-L-rhamnosidase competitively. Tetrahedron: Asymmetry, 20(20): 2368-2373.

Muller-Domboa, D., Ellenberg, H. 1974: Aims and methods of vegetation ecology, John Wiley & Sons, Ltd., NY.

Nash, R. J., Kato, A., Yu, C. Y., Fleet, G. W. 2011: Iminosugars as therapeutic agents: recent advances and promising trends. Future Medicinal Chemistry, 3(12): 1513-1521.

Papoutsi, Z., Kassi, E., Halabalaki, M., Mitakou, S., Moutsatsou, P. 2007: Evaluation of estrogenic/antiestrogenic activity of Onobrychis ebenoides extract–Interaction with estrogen receptor subtypes ERα and ERβ. Toxicology in vitro 21(3): 364-370.

Petrova, A., Vladimirov. V. (eds). 2008: Red List of Bulgarian vascular plants. Phytologia Balcanica, 15 (1): 63-94.

Pieroni, A., Giusti, M. E. 2009: Alpine ethnobotany in Italy: traditional knowledge of gastronomic and medicinal plants among the Occitans of the upper Varaita valley, Piedmont. Journal of Ethnobiology and Ethnomedicine 5(1): 32-35.

Roussakova, V. 2015: in Biserkov V (ed.) Red Data Book of the Republic of Bulgaria Volume 3 - Natural habitats. Joint edition of the Bulgarian Academy of Sciences & Ministry of Environment and Water, Sofia.

Tchokouaha, R. F., Alexi, X., Chosson, E., Besson, T., Skaltsounis, A.L., Seguin, E., Wandji, J. 2010: Erymildbraedin A and B, two novel cytotoxic dimethylpyrano-isoflavones from the stem bark of Erythrina mildbraedii: evaluation of their activity toward endocrine cancer cells. Journal of enzyme inhibition and medicinal 20] Winchester BG (2009) Iminosugars: from botanical curiosities to licensed drugs. Tetrahedron: Asymmetry 20(6): 645-651.

Velchev, V. (ed.) 1992: Atlas of the endemic plants in Bulgaria. Sofia.

Wester, P., Lunau, K. 2016: Plant–Pollinator Communication. Advances in Botanical Research 81 (in press),


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