Role of 11β-hydroxysteroid dehydrogenase gene polymorphisms rs846910 and rs12086634 in the etiology of hepatocellular carcinoma

Ahmed Mudher Al-Khaykanee, Soura Alaa Hussein, Z Muhsen M Ali

Abstract


In Asia, the most frequent cause of hepatocellular carcinoma (HCC) is chronic hepatitis B. The hepatitis B virus's (HBV) genomic integration is probably a precursor to carcinogenesis. The integrated HBV genome may directly activate nearby cellular genes to give the liver cells a selective growth advantage. Our study's goal was to determine if genetic variants in 11β-HSD1, rs846910 and rs12086634 could be linked to chronic hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). 150 samples were collected from people suffering from hepatitis B with HCC, HCC, and healthy people. The samples were genotyped for 11β HSD1 (rs846910 and rs12086634) using allele-specific real-time PCR. After performing genotyping of the 11β-HSD1 gene using real-time PCR, both alleles G and A of rs846910 were found to be more prevalent in the two groups of hepatocellular carcinoma and hepatitis B. This gives a clear indication about the possibility of contracting hepatocellular carcinoma and hepatitis B. A different result was found than previously, as the TT genotype of rs12086634 showed a protective effect against hepatocellular carcinoma and hepatitis B virus infection, while the AG genotype of rs846910 increased the risk of hepatocellular carcinoma. These genetic variations are clearly significant biomarkers for hepatocellular carcinoma.


Keywords


single nucleotide polymorphism, 11β HSD1, Hepatocellular carcinoma (HCC)

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References


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