Nerium oleander L.: A review of diversity, toxicity, chemical compositions and biological activities
Abstract
The Apocynaceae family includes the evergreen ornamental shrub known as oleander (Nerium oleander). Numerous cultivars were listed in this species, and these differ essentially by the color of the flower. The level of diversity within this species is linked to several factors, such as the geographical region where it grows naturally, environmental conditions, and genetic diversity. Numerous studies of this plant have highlighted bioactive ingredients and several pharmacological effects. Nerium oleander is an extremely toxic and even fatal plant because cardiac glycosides such as oleandrin and neriin are constituents present in different parts of the plant. However, a few common oleander pests occasionally feed on the bush. Despite the danger, oleander is of great medicinal importance. Compounds such as terpenes, steroids, polyphenols, and flavonoids have been identified in extracts of different plant parts. The essential oil also includes compounds such as digitoxigenin. Its compounds are involved in several biological activities such as anti-inflammatory, antioxidant, anticancer, hepatoprotective, antimicrobial, larvicidal, and antidiabetic activities which can be used to produce natural drugs.
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Aboud, A. S. (2015). Antimicrobial activities of aqueous and ethanolic extracts from Nerium oleander used in the treatment of burns infections isolates. Journal of Pharmacy, Chemistry and Biological Sciences, 2(4), 248-258.
Al-Ansi, A., Dhafer, H. A., Al-Tamimi, J., & Murshed, M. (2024). Evaluation of Nerium oleander extracts against Pachycondyla sennaarensis (Hymenoptera, Formicidae) adults. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 76(6), e13189. https://doi.org/10.1590/1678-4162-13189
Al-Snafi, A. E. (2020). Bioactive ingredients and pharmacological effects of Nerium oleander. IOSR Journal of Pharmacy, 10(9), 19-32.
Al-Snai, A., Mousa, H., & Majid, W. J. (2019). Medicinal plants possessed hepatoprotective activity. IOSR Journal of Pharmacy, 9(8), 26-56.
Ali, H. F. M., El-Ella, F. M. A., & Nasr, N. F. (2010). Screening of chemical analysis, antioxidant antimicrobial and antitumor activities of essential oil of oleander (Nerium oleander) flower. International Journal of Biological Chemistry, 4(4), 190-202.
Almanaa, T. N., Alharbi, N. S., Ramachandran, G., Chelliah, C. K., Rajivgandhi, G., Manoharan, N., Kadaikunnan, S., Khaled, J. M., & Alanzi, K. F. (2021). Anti-biofilm effect of Nerium oleander essential oils against biofilm-forming Pseudomonas aeruginosa on urinary tract infections. Journal of King Saud University-Science, 33(2), 101340.
Atay Balkan, İ., Gören, A. C., Kırmızıbekmez, H., & Yeşilada, E. (2018). Evaluation of the in vitro anti-inflammatory activity of Nerium oleander L. flower extracts and activity-guided isolation of the active constituents. Records of Natural Products, 12(2): 128-141.
Bagari, M., Bouhaimi, A., Ghaout, S., & Chihrane, J. (2013). The toxic effects of Nerium oleander on larvae of the desert locust Schistocerca gregaria (Forskål, 1775) (Ortoptera, Acrididae). Zoologica Baetica, 24(1), 193-203.
Barai, A. C., Paul, K., Dey, A., Manna, S., Roy, S., Bag, B. G., & Mukhopadhyay, C. (2018). Green synthesis of Nerium oleander-conjugated gold nanoparticles and study of its in vitro anticancer activity on MCF-7 cell lines and catalytic activity. Nano Convergence, 5(1), 1-9.
Barbouche, N., Hajjem, B., Lognay, G., & Ammar, M. (2001). Contribution à l’étude de l’activité biologique d’extraits de feuilles de Cestrum parqui L’Hérit. (Solanaceae) sur le criquet pèlerin Schistocerca gregaria (Forsk.). Biotechnology, Agronomy, Society and Environment, 5(2), 85-90.
Bi, S., Zhu, G., Wu, J., Li, Z., Fang, L., & Wu, H. (2016). Composition and antioxidant activities of the essential oil from the flowers of Nerium indicum. Chemistry of Natural Compounds, 52, 1098-1099.
Cao, L., Si, J. Y., Liu, Y., Sun, H., Jin, W., Li, Z., Zhao, X. H., & Le Pan, R. (2009). Essential oil composition, antimicrobial and antioxidant properties of Mosla chinensis Maxim. Food Chemistry, 115(3), 801-805.
Dardona, A. W. Y., & Shahabuddin, D. (2022). Evaluation of antimicrobial activity of methanolic and ethanolic extracts of three varieties of Nerium oleander. Journal of Pharmaceutical Negative Results, 1821-1829.
Demirel Kars, M., Odabaşı, B. A., Kars, G., Üney, K., Bağcı, Y., & Baş, A. L. (2014). Implications from a pharmacogenomic analysis: Nerium oleander leaf distillate supplemented diet regulates cholesterol metabolism in rats. Pharmaceutical Biology, 52(8), 988-993.
El Sawi, N. M., Geweely, N. S., Qusti, S., Mohamed, M., & Kamel, A. (2010). Cytotoxicity and antimicrobial activity of Nerium oleander extracts. Journal of Applied Animal Research, 37(1), 25-31.
El-Akhal, F., Guemmouh, R., Ez Zoubi, Y., & El Ouali Lalami, A. (2015). Larvicidal activity of Nerium oleander against larvae West Nile vector mosquito Culex pipiens (Diptera: Culicidae). Journal of Parasitology Research, 2015, 943060.
El-Sayed, S. H., & El-Bassiony, G. M. (2016). Larvicidal, biological and genotoxic effects, and temperature-toxicity relationship of some leaf extracts of Nerium oleander (Apocynaceae) on Culex pipiens (Diptera: Culicidae). Journal of Arthropod-Borne Diseases, 10(1), 1-11.
El-Shazly, M. M., Nassar, M. I., & El-Sherief, H. A. (1996). Toxic effect of ethanolic extract of Nerium oleander (Apocynaceae) leaves against different developmental stages of Muscina stabulans (Diptera-Muscidae). Journal of the Egyptian Society of Parasitology, 26(2), 461-473.
Erdemoglu, N., Küpeli, E., & Yeşilada, E. (2003). Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. Journal of Ethnopharmacology, 89(1), 123-129.
Farooqui, S., & Tyagi, T. (2018). Nerium oleander: Its application in basic and applied science: A review. International Journal of Pharmacy and Pharmaceutical Sciences, 10(3), 1-4.
Garima, Z., & Amla, B. (2010). A review on chemistry and pharmacological activity of Nerium oleander L. Journal of Chemical and Pharmaceutical Research, 2(6), 351–358.
Gayathri, V., Ananthi, S., Chandronitha, C., Ramakrishnan, G., Sundaram, R. L., & Vasanthi, H. R. (2011). Cardioprotective effect of Nerium oleander flower against isoproterenol-induced myocardial oxidative stress in experimental rats. Journal of Cardiovascular Pharmacology and Therapeutics, 16(1), 96-104.
Gayathri, V., Ananthi, S., & Vasanthi, H. R. (2013). Antihyperlipidemic potential of polyphenol and glycoside-rich Nerium oleander flower against triton WR-1339-induced hyperlipidemia in experimental Sprague Dawley rats. Journal of Chemistry, 2013, 825290.
Gunes, A., Guler, E., Un, R. N., Demir, B., Barlas, F. B., Yavuz, M., Coskunol, H., & Timur, S. (2017). Niosomes of Nerium oleander extracts: In vitro assessment of bioactive nanovesicular structures. Journal of Drug Delivery Science and Technology, 37, 158-165.
Hadizadeh, I., Peivastegan, B., & Kolahi, M. (2009). Antifungal activity of nettle (Urtica dioica L.), colocynth (Citrullus colocynthis L. Schrad), oleander (Nerium oleander L.), and konar (Ziziphus spina-christi L.) extracts on plant pathogenic fungi. Pakistan Journal of Biological Sciences, 12(1), 58-63.
Hameed, I. H., Jasim, H., Kareem, M. A., & Hussein, A. O. (2015). Alkaloid constitution of Nerium oleander using gas chromatography-mass spectroscopy (GC-MS). Journal of Medicinal Plants Research, 9(9), 326-334.
Harizia, A., & Doumandji, S. (2014). Biological activity of Nerium oleander L. (Apocynaceae) essential oil on the 5th larval stage of Schistocerca gregaria (Forskål, 1775) (Orthoptera: Acrididae). International Journal of Zoology and Research, 4(6), 19-28.
Havlik, J., Budesinsky, M., Kloucek, P., Kokoska, L., Valterova, I., Vasickova, S., & Zeleny, V. (2009). Norsesquiterpene hydrocarbon, chemical composition and antimicrobial activity of Rhaponticum carthamoides root essential oil. Phytochemistry, 70(3), 414-418.
Hussain, M. A., & Gorsi, M. S. (2004). Antimicrobial activity of Nerium oleander Linn. Asian Journal of Plant Sciences, 3(2), 177-180.
Jeyachandran, R., Baskaran, X., & Cindrella, L. (2010). Screening of phytochemical and antibacterial potential of four Indian medicinal plants. Libyan Agricultural Research Center Journal International, 1(5), 301-306.
Ling, Y., Yu, J., Huang, Y., Chen, M., & Zhang, Q. (2025). Identification of the chemical constituents in the leaves and twigs of Nerium oleander by ultrahigh‐performance liquid chromatography coupled to quadrupole Orbitrap high‐resolution mass spectrometry. Rapid Communications in Mass Spectrometry, 39(2), e9942.
Madaci, B., Merghem, R., Doumandji, B., & Soltani, N. (2008). Effet du Nerium oleander, laurier-rose, (Apocynaceae) sur le taux des protéines, l'activité de l'AChE et les mouvements des vers blancs Rhizotrogini (Coleoptera Scarabaeidae). Sciences & Technologie. C, Biotechnologies, 0(27), 73-78.
Malik, R., Bokhari, T. Z., Siddiqui, M. F., Younis, U., Hussain, M. I., & Khan, I. A. (2015). Antimicrobial activity of Nerium oleander L. and Nicotiana tabacum L.: A comparative study. Pakistan Journal of Botany, 47(4), 1587-1592.
Marchese, A., Orhan, I. E., Daglia, M., Barbieri, R., Di Lorenzo, A., Nabavi, S. F., Gortzi, O., Izadi, M., & Nabavi, S. M. (2016). Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chemistry, 210, 402-414.
Mary, S. J., Chithra, B., & Sivajiganesan, S. (2017). In vitro anti-inflammatory activity of the flowers of Nerium oleander (white). International Journal of Research in Granthaalayah, 5, 123-128.
Minnat, T. R. (2016). In vivo and in vitro antibacterial assessment of Nerium oleander aqueous leaf extract against bacterial pathogens and its effect in treatment of wounds. AL-Qadisiyah Journal of Veterinary Medical Sciences, 15(2), 31-39.
Mohadjerani, M. (2012). Antioxidant activity and total phenolic content of Nerium oleander L. grown in North of Iran. Iranian Journal of Pharmaceutical Research, 11(4), 1121.
Mouhcine, M., Amin, L., Saaid, A., Khalil, H., & Laila, B. (2019). Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco. Asian Pacific Journal of Tropical Medicine, 12(1), 32-37.
Namian, P., Talebi, T., Germi, K. G., & Shabani, F. (2013). Screening of biological activities (antioxidant, antibacterial, and antitumor) of Nerium oleander leaf and flower extracts. Vacuum, 10(11), 378-384.
Nawaz, Z., Khalid, H. N., Sajid, A., Arshed, F., Ahmed, E., Sharif, A., Khan, I. H., Javaid, A., & Sajid, A. (2023). A new bioactive steroid isolated from Nerium oleander L. International Journal of Biology and Biotechnology, 20(1), 31-36.
Newman, R. A., Kondo, Y., Yokoyama, T., Dixon, S., Cartwright, C., Chan, D., Johansen, M., & Yang, P. (2007). Autophagic cell death of human pancreatic tumor cells mediated by oleandrin, a lipid-soluble cardiac glycoside. Integrative Cancer Therapies, 6(4), 354-364.
Newman, R. A., & Yang, P. (2015). Response to: Does the Nerium oleander extract PBI-05204 have potential for pancreatic cancer? Investigational New Drugs, 33, 788-789.
Ormancey, X. (2001). Formulation of essential oils in functional perfumery. Parfums, Cosmetiques, Actualites, 157, 30-40.
Page, R. D. M. The Plant List with literature. Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow. Checklist dataset https://doi.org/10.15468/btkum2 accessed via GBIF.org on 2025-03-13.
Pan, Y., Rhea, P., Tan, L., Cartwright, C., Lee, H.-J., Ravoori, M. K., Addington, C., Gagea, M., Kundra, V., & Kim, S. J. (2015). PBI-05204, a supercritical CO₂ extract of Nerium oleander, inhibits growth of human pancreatic cancer via targeting the PI3K/mTOR pathway. Investigational New Drugs, 33, 271-279.
Patel, S. (2016). Plant-derived cardiac glycosides: Role in heart ailments and cancer management. Biomedicine & Pharmacotherapy, 84, 1036-1041.
Rashan, L. J., Franke, K., Khine, M. M., Kelter, G., Fiebig, H. H., Neumann, J., & Wessjohann, L. A. (2011). Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum. Journal of Ethnopharmacology, 134(3), 781-788.
Raveen, R., Kamakshi, K. T., Deepa, M., Arivoli, S., & Tennyson, S. (2014). Larvicidal activity of Nerium oleander L. (Apocynaceae) flower extracts against Culex quinquefasciatus Say (Diptera: Culicidae). International Journal of Mosquito Research, 1(1), 38-42.
Regnault-Roger, C., Hamraoui, A., Holeman, M., Theron, E., & Pinel, R. (1993). Insecticidal effect of essential oils from Mediterranean plants upon Acanthoscelides obtectus Say (Coleoptera, Bruchidae), a pest of kidney bean (Phaseolus vulgaris L.). Journal of Chemical Ecology, 19, 1233-1244.
Roni, M., Murugan, K., Panneerselvam, C., Subramaniam, J., & Hwang, J.-S. (2013). Evaluation of leaf aqueous extract and synthesized silver nanoparticles using Nerium oleander against Anopheles stephensi (Diptera: Culicidae). Parasitology Research, 112, 981-990.
Sacchetti, G., Maietti, S., Muzzoli, M., Scaglianti, M., Manfredini, S., Radice, M., & Bruni, R. (2005). Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemistry, 91(4), 621-632.
Saeed, H. M., Ferdosi, M. F. H., Khan, I. H., Javaid, A., & Sultan, M. W. (2023). Antibacterial activity and GC-MS analysis of white flowers extract of Nerium oleander L. International Journal of Biology and Biotechnology, 20(1), 163-168.
Sanna, G., Madeddu, S., Serra, A., Collu, D., Efferth, T., Hakkim, F. L., & Rashan, L. (2021). Anti-poliovirus activity of Nerium oleander aqueous extract. Natural Product Research, 35(4), 633-636.
Saranya, S., Archana, D., Santhy, K., Saranya, S., Archana, D., & Santhy, K. S. (2017). Antimicrobial and antioxidant effects of Nerium oleander flower extracts. International Journal of Current Microbiology and Applied Sciences, 6(5), 1630-1637.
Sawamura, M. (2000). Aroma and functional properties of Japanese yuzu (Citrus junos Tanaka) essential oil. Aroma Research, 1(1), 14-19.
Sedaghat, M. M., Dehkordi, A. S., Abai, M. R., Khanavi, M., Mohtarami, F., Abadi, Y. S., Rafi, F., & Vatandoost, H. (2011). Larvicidal activity of essential oils of Apiaceae plants against malaria vector, Anopheles stephensi. Iranian Journal of Arthropod-Borne Diseases, 5(2), 51.
Siddiqui, I., Bokhari, N. A., & Perveen, K. (2016). Antifungal ability of Nerium oleander against Fusarium oxysporum, Sclerotium rolfsii and Macrophomina phaseolina. Journal of Animal and Plant Sciences, 26(1), 269-274.
Singh, S., Shenoy, S., Nehete, P. N., Yang, P., Nehete, B., Fontenot, D., Yang, G., Newman, R. A., & Sastry, K. J. (2013). Nerium oleander-derived cardiac glycoside oleandrin is a novel inhibitor of HIV infectivity. Fitoterapia, 84, 32-39.
Singhal, K. G., & Gupta, G. (2012). Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl₄–induced liver injury in rats. Asian Pacific Journal of Tropical Medicine, 5(9), 677-685.
Singhal, K. G., & Gupta, G. (2011). Some central nervous system activities of Nerium oleander Linn (Kaner) flower extract. Tropical Journal of Pharmaceutical Research, 10(4), 455-461.
Sinha, S. N., & Biswas, K. (2016). A concise review on Nerium oleander L.-An important medicinal plant. Tropical Plant Research, 3, 408-412.
Shashikala, G. H., Shilpa, B. N., & Shah, M. J. (2018). Evaluation of anxiolytic activity of aqueous extract of Nerium oleander flowers in albino rats. International Journal of Basic & Clinical Pharmacology, 7(9), 1797-1802. https://doi.org/10.18203/2319-2003.ijbcp20183492
Sun, J. (2007). D-Limonene: Safety and clinical applications. Alternative Medicine Review, 12(3), 259.
Wang, X., Plomley, J. B., Newman, R. A., & Cisneros, A. (2000). LC/MS/MS analyses of an Nerium oleander extract for cancer treatment. Analytical Chemistry, 72(15), 3547-3552.
Zaid, R., Canela-Garayoa, R., Ortega-Chacón, N. M., & Mouhouche, F. (2022). Phytochemical analyses and toxicity of Nerium oleander (Apocynaceae) leaf extracts against Chaitophorus leucomelas Koch, 1854 (Homoptera: Aphididae). Journal of the Saudi Society of Agricultural Sciences, 21(5), 310-317.
Zhai, J., Dong, X., Yan, F., Guo, H., & Yang, J. (2022). Oleandrin: A systematic review of its natural sources, structural properties, detection methods, pharmacokinetics, and toxicology. Frontiers in Pharmacology, 13, 822726. https://doi.org/10.3389/fphar.2022.822726
Zhao, M., Zhang, S., Fu, L., Li, N., Bai, J., Sakai, J., Wang, L., Tang, W., Hasegawa, T., & Ogura, H. (2006). Taraxasterane-and ursane-type triterpenes from Nerium oleander and their biological activities. Journal of Natural Products, 69(8), 1164-1167.
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