Chenopodium rubrum L. as a model plant for physiological and biochemical investigations of ontogenesis in vitro

Abstract

Mitrović, A., Živanović, B., Dučić, T., Bogdanović-Pristov, J., Radotić Hadži-Manić, K.: Chenopodiumrubrum L. as a model plant for physiological and biochemical investigations of ontogenesis in vitro.Biologica Nyssana, 1 (1-2), December 2010: 61-64.

Chenopodium rubrum L. , is a suitable model plant for studying ontogenesis in vitro as an early floweringspecies. Culture of intact plants in vitro and antioxidative enzymes detection were performed. Growth patternto the end of ontogenesis, flowering and seed development are all determined by the photoperiod seedlingsexperience during induction and evocation of flowering. Different phases of vegetative and reproductivedevelopment are characterized by changes in antioxidative enzymes activities. We showed sequentialexpression of antioxidative enzymes during seed germination. Prior to radicule protrusion, CAT and SODshowed maximal activity, while POD activity appeared later. The highest catalase (CAT) activity wasmeasured at the time of flowering while peroxidases (PODs) are involved in determination of growth anddevelopment in accordance with the environmental clues. The absence of some superoxide dismutase (SOD)isoforms could be the indicator of senescence. Seed ageing affect changes in antioxidative status of seeds,germination, seedling growth and flowering.

Key words: catalase, Chenopodium rubrum, germination, flowering, peroxidase, superoxide dismutase