Somatic embryogenesis and in vitro plantlet regeneration of Lilium martagon L. var. cattaniae Vis.

Abstract

Karalija, E., Trbojević, S., Parić, A.: Somatic embryogenesis and in vitro plantlet regeneration of Liliummartagon L. var. cattaniae Vis.. Biologica Nyssana, 1 (1-2), December 2010: 57-60.

In this study organogenic capacity of two different explants type (leaves and whole bulbs) of Liliummartagon L. var. cattaniae Vis. was examined. For induction of in vitro somatic embryogenesis andadventitive regeneration different concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzilaminopurine(from 0,25 mg/l to 8,00 mg/l) added to MS basal medium were used. Our results indicate that concentrationof 0,5 mg/l 2,4-dichlorophenoxyacetic acid and 4 mg/l 6- benzilaminopurine promoted somaticembryogenesis from leaves of Lilium martagon var. cattaniae, while all other concentrations promoted directshoot regeneration from bulb explants. Root formation was induced on MS basal medium with 0,2 mg/lindole butyric acid. These plantlets were acclimatized well in a greenhouse conditions.

Key words: Lilium martagon var. cattaniae, somatic embryogenesis, regeneration