Effect of different extraction solvents on total phenolic and flavonoid contents and antioxidant activity of the Bolboschoenus laticarpus rhizome extracts

Danijela Nikolić, Andrea Žabar Popović, Milica Vidanović, Dragana Jenačković Gocić, Perica Vasiljević, Marina Jušković

Abstract


The aim of this study was to determine and compare antioxidant activity and total phenol and flavonoid content in various rhizome extracts of Bolboschoenus laticarpus. Methanolic, ethanolic, chloroform, and ethyl acetate extracts were tested at 250, 500, 625, 750, 825, and 1,000 µg/ml of dry extract concentrations. The antioxidant activity of the extracts was evaluated using the DPPH method, the total flavonoid content by the aluminium chloride (AlCl3) method and the total phenolic content by the Folin-Ciocalteau method. The results revealed that ethanolic extract had the highest antioxidant capacity (IC50, 0.981 mg/ml) while the chloroform extract showed the lowest activity (IC50, 11.78 mg/ml). Analysis of total phenolic content in different extract types showed that ethyl acetate extract had the highest concentration of phenols while chloroform extracts had the lowest values. The highest concentration of flavonoids was noticed in ethyl acetate extract while the lowest concentration was determined in methanolic extract. This study confirmed that rhizome of B. laticarpus has significant antioxidant capacity and is a good source of phenols and flavonoids.


Keywords


Bolboschoenus laticarpus, different solvents, antioxidant activity, phenols, flavonoids

Full Text:

PDF

References


Anokwuru, C.P., Anyasor, G.N., Ajibaye, O., Fakoya, O., & Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Nature and Science, 9(7), 53-61.

Blois, M. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181, 1199–1200. https://doi.org/10.1038/1811199a0

Browning, J., Gordon-Gray, K.D., Smith, S.G., & Van Staden, J. (1996). Bolboschoenus yagara (Cyperaceae) newly reported for Europe. Annales Botanici Fennici, 33, 129-136.

Cao, G., Sofic, E., & Prior, R. L. (1997). Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free radical biology and medicine, 22(5), 749-760.

Chang, C.C., Yang, M.H., Wen, H.M., & Chern, J.C. (2002). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3), 178-182.

Dai, J. & Mumper, R.J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313-7352.

Dapkevicius, A., Venskutonis, R., van Beek, T.A., & Linssen, J.P. (1998). Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture, 77(1), 140-146.

Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., & Ju, Y.H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296-302.

Ewing, K. (1983). Environmental controls in Pacific Northwest intertidal marsh plant communities. Canadian Journal of Botany, 61, 1105–1116.

Ghasemzadeh, A., Jaafar, H.Z., & Rahmat, A. (2010). Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules, 15(6), 4324–33.

Grigore, M.N. & Oprica, L. (2015). Halophytes as possible source of antioxidant compounds, in a scenario based on threatened agriculture and food crisis. Iranian Journal of Public Health, 44(8), 1153-5.

Hroudová, Z., Zákravský, P., Duchaček, M., & Marhold, K. (2007). Taxonomy distribution and ecology of Bolboschoenus in Europe. Annales Botanici Fennici, 44, 81-102.

Hroudová, Z., Zákravský, P., & Frantik, T. (1999). Ecological differentiation of Central European Bolboschoenus taxa and their relationship to plant communities. Folia Geobotanica 34, 77–96.

Jakopic, J., Veberic, R., & Stampar, F. (2009). Extraction of phenolic compounds from green walnut fruits in different solvents. Acta Agriculturae Slovenica, 93(1), 11-15.

Jarolimova, V. & Hroudová, Z. (1998). Chromosome numbers within the genus Bolboschoenus in Central Europe. Folia Geobotanica, 33, 415-428.

Mahboubi, M., Kazempour, N., & Boland Nazar, A.R. (2013). Total phenolic, total flavonoids, antioxidant and antimicrobial activities of Scrophularia striata Boiss. extracts. Jundishapur Journal of Natural Pharmaceutical Products 8(1), 15-9.

Marhold, K., Hroudová, Z., Duchaček, M., & Zákravský P. (2004). The Bolboschoenus maritimus group (Cyperaceae) in central Europe, including B. laticarpus, spec. nova. Phyton, 44, 1-21.

Mikulka, J., Chodova, D., & Abrahamova, I. (1999). Expandujici Kamyšnik primorsky (Bolboschoenus maritimus (L.) Palla) na orne pude. Farmar, 11, 27-28.

Nikolić, D., Gocić, D. J., Jušković, M., & Ranđelović, V. (2019). Morphological differentiation of populations of Bolboschoenus taxa in Serbia. Plant Biosystems - An International Journal Dealing with All Aspects of Plant Biology, 154(4), 488–502. https://doi.org/10.1080/11263504.2019.1651772

Popescu, A., Pavalache, G., Pirjol, T. N., & Istudor, V. (2016). Antioxidant comparative activity and total phenolic content of Scirpus holoschoenus L. (Holoschoenus vulgaris Link) depending on extraction condition and the solvent used. Revista de Chimie, 67(2), 255-259.

Powell, R.G., Bajaj, R., & McLaughlin, J.L. (1987). Bioactive stilbenes of Scirpus maritimus. Journal of Natural Products, 50, 293.

Simpson, D.A. & Inglis, C.A. (2001). Cyperaceae of economic, ethnobotanical and horticultural importance: a checklist. Kew Bulletin, 257-360.

Singleton, V.L., Orthofer, R., & Lamuela-Raventos, R.M. (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.

Sultana, B., Anwar, F., & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14(6), 2167-2180.

Turkmen, N., Sari, F., & Velioglu, Y.S. (2006). Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods. Food chemistry, 99(4), 835-841.

Yu, J., Ahmedna, M., & Goktepe, I. (2005). Effects of processing methods and extraction solvents on concentration and antioxidant activity of peanut skin phenolics. Food Chemistry, 90, 199–206.

Zhou, K. & Yu, L. (2004). Effects of extraction solvent on wheat bran antioxidant activity estimation. Lebennsmittel Wissenschaft und Technologie, 37, 717–721.


Refbacks

  • There are currently no refbacks.