Antimicrobial activity of Evernia prunastri (оakmoss) resinoids

Svetlana Bogdanović, Ivana Zlatković, Dobrila Ranđelović


Infections caused by strains of bacteria that show resistance to a large number of antibiotics represent one of the leading problems today. The aim of this work is to examine new, natural resources with potential antimicrobial effects. The antibacterial activity of lichen resinoids (Evernia prunastri) was tested against reference strains Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and clinical isolates of bacteria S. aureus, S. epidermidis, P. aeruginosa and E. coli, by the disc-diffusion method. Each 10 µl of resinoid, of different concentrations, was applied to sterile discs with a diameter of 6 mm. The tested resinoid exhibited the most significant antibacterial activity against Pseudomonas aeruginosa ATCC 27853, while it was the weakest against the clinical isolate of Escherichia coli. The results indicate that the tested oakmoss resinoid shows moderate antimicrobial activity against the tested strains.


antimicrobial activity, resinoid, lichen, bacteria

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Aslan A., Gulluce M., Sokmen M., Adiguzel A., Sahin F., Ozkan H. 2006: Antioxidant and antimicrobial properties of the lichens Cladonia foliacea, Dermatocarpon miniatum, Evernia divaricata, Evernia prunastri and Neofuscella pulla. Pharmaceut. Biol. 44: 247-252.

Bobbarala, V. (2012): Antimicrobial Agents. - Intech, ISBN 978-953-51-0723-1.

Calcott M.J., Ackerley D.F., Knight A. 2018: Secondary metabolismin the lichen symbiosis. Chem Soc Rev 47:1730–1760.

Chahra D., Ramdani M., Lograda T., Chalard P., Figueredo G. 2016: Chemical composition and antimicrobial activity of Evernia prunastri and Ramalina farinacea from Algeria. Biological Sciences and Pharmaceutical Research, 4:35-42.

Crawford SD. 2015: Lichens used in traditional medicine. In: Ranković B (ed) Lichen secondary metabolites: bioactive properties and pharmaceutical potential. Springer International Publishing, Cham, pp 27–80.

Grundmann H., de Kraker M., Davey P. 2011: Clinical impact of antimicrobial resistance: design matters. Lancet Infect Dis, 11:344.

Joulain D., Tabacchi R. 2009: Lichen extracts as raw materials in perfumery. Part 1: oakmoss. Flavour Fragr J 24:49–61.

Kahriman N., Yazici K., Arslan T., Aslan A., Karaoglu SA., Yayli N. 2011: Chemical composition and antimicrobial activity of the essential oils from Evernia prunastri (L.) ach. and Evernia divaricata (L.) ach. Asian Journal of Chemistry 23:1937-1939.

Nash TH. 2008: Lichen Biology, 2nd ed. Cambridge: Cambridge University Press.

Shcherbakova A., Strömstedt A., Göransson U., Gnezdilov O., Turanov A., Boldbaatar D., Kochkin D., Ulrich Merzenich G., Koptina A. 2021: Antimicrobial and antioxidant activity of Evernia prunastri extracts and their isolates.World Journal of Microbiology and Biotechnology 37:129.

Stojanović I., Radulović N., Cvetković V., Mitrović T., Stamenković S. 2013: Antimicrobial activity of methanol extracts of four Parmeliaceae lichen species. FACTA UNIVERSITATIS Series: Physics, Chemistry and Technology 11:45 – 53. DOI: 10.2298/FUPCT1301045S

Studzinska-Sroka E., Galanty A., Bylka W. 2017: Atranorin - an interesting lichen secondary metabolite. Mini Rev Med Chem 17:1633–1645.

Tenover FC. 2006: Mechanisms of antimicrobial resistance in bacteria. American Journal of Infection Control 34. doi: 10.1016/j.amjmed.2006.03.011.


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