Phylogenetic relationships of some Worldwide-cultivated cotton genotypes (Gossypium hirsutum L.) using ISSR and RAPD markers

Abstract

Cotton, which belongs to the Gossypium genus of the Malvaceae family and economically and biologically highly valuable multi-purpose crop grown as a source of fiber, food and feed worldwide. In this study, phylogenetic relationships of cotton genotypes of thirteen countries (twelve Gossypium hirsutum and one Gossypium barbadense (GIZA 75 Egypt) as outgroup) taken from the Directorate of Nazilli Cotton Research Institute genetic stock was analyzed by using RAPD and ISSR markers.For RAPD –PCR analysis showed that in the highest bands OPA-02 and OPA-13 primers, no band was obtained from OPE-08 primer and total of 53 DNA bands were obtained and rate of polymorphism was about 90.56%. For ISSR –PCR analysis showed that in the highest bands UBC-811, UBC-834 and UBC-836 primers, no band was obtained from UBC-853 primer and total of 81 DNA bands were obtained and rate of polymorphism was about 77.77%. Both RAPD and ISSR analysis, phylogenetic tree was obtained by using the UPGMA algorithm and the tree consisted of two large clades. Analysis of the results suggests that RAPD and ISSR markers are useful tools to demonstrate phylogenetic relationships between cotton genotypes.