Evaluation of antioxidant, antimicrobial and antibiofilm activities of Fomes fomentarius (L.) Fr.

Eylül İrem İrez, Nurcihan Hacioğlu Doğru, Neslihan Demir


This paper was evaluated antioxidant, antimicrobial and antibiofilm activities of ethanol, methanol, acetone and chloroform extracts of Fomes fomentarius (L.) Fr. Antioxidant activity was evaluated by using 2,2-diphenyl-1-picrylhydrazyl. The antimicrobial screenings were carried out via disc diffusion method, minimal inhibition concentration and minimal bactericidal concentration against eight test microorganisms: Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, S. haemolyticus ATCC 43252 and Candida albicans ATCC 10231. In vitro antibiofilm activities of extracts were also investigated based on crystal violet binding assay against test microorganisms. All the extracts tested had higher antioxidant activity compared with butylated hydroxytoluene which is a used as a standard. Ethanol, methanol and acetone extracts of macrofungi also showed higher antimicrobial effect against P. aeruginosa ATCC 27853 than comparison antibiotic P10. The lowest minimal inhibition concentration value was recorded by F. fomentarius ethanol extract against S. haemolyticus ATCC 43252. The highest antibiofilm activity was  also noticed against biofilm formed by S. haemolyticus ATCC 43252. The results indicated that the F. fomentarius tested may be potential sources for isolation of natural antioxidant, antimicrobial and antibiofilm compounds.


antioxidant; antimicrobial; antibiofilm activity; Fomes fomentarius (L.) Fr.

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