Antioxidant phenolic compounds in Hypericum perforatum L. wild-growing plants collected in the Republic of Macedonia

Oliver Tusevski, Marija Todorovska, Mirko Spasenoski, Sonja Gadzovska Simic


The aim of this study was to evaluate phenolic compounds composition and antioxidant activity in roots (RO), non-flowering shoots (NFS) and flowering shoots (FS) of Hypericum perforatum L. wild-growing plants collected in the Republic of Macedonia. The analyses of total phenolic compounds included quantification of phenolics, flavonoids, catechin derivatives, flavan-3-ols, condensed tannins and hypericins. Antioxidant activity was determined by the cupric ions reducing antioxidant capacity, phosphomolybdenum test, reducing power and DPPH scavenging. The contents of phenolics in FS and NFS were significantly higher than those found in RO extracts. Hypericins content in FS was 2-fold increased in comparison to NFS, while RO showed low capability for accumulation of naphthodianthrones. Also, FS and NFS exhibited markedly higher antioxidant activities compared to RO. In summary, aerial parts of H. perforatum accumulated significant levels of antioxidant phenolic compounds that were characterized with hydrogen atom donation, radical scavenging and participation in redox reactions.


Antioxidant activity; Flowering shoots; Hypericum perforatum L.; Non-flowering shoots; Phenolic compounds; Roots.

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