Effects of salt stress factors on antimicrobial activity of two Triticum aestivum L. varieties

Nurcihan Hacıoğlu Doğru, Okan Acar

Abstract


Salinity is one of the most common environmental stress factors that adversely affect plant growth and crop production in cultivated areas worldwide. Herbal or ‘alternative’ medicine is gaining popularity and scientific research about wheat grass as a “functional food” is becoming more available and popular. Wheat grass, Triticum aestivum L. has a long history and is widely used as a health food supplement. It is found to be used as a treatment for minor ailments and serious life threatening issues, and also as a preventative dietary supplement and therapeutic drugs. Current study was aimed at evaluation of antimicrobial properties of two varieties of T. aestivum L. [cv. Tosunbey (drought tolerant) and cv. Sultan 95 (drought sensitive)] which grown in three different condition [(1) control; not treatment with salt or acetyl salicylic acid; (2) treatment with sea water; (3) sea water and pre-treatment of seeds with acetyl salicylic acid]. The antimicrobial activity of the ethanol extracts of two varieties of T. asetivum were assayed against Escherichia coli NRRL B-3704, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 13315, Acinetobacter baumanii ATCC 19606, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, S. haemolyticus ATCC 43252 and Candida albicans ATCC 10231 test microorganisms by agar disc diffusion method and micro broth dilution methods. The results of showed that the ethanol extracts from the different treatments studied showed antimicrobial activities, with the diameters of the inhibition zone ranging from 8 to 13 mm and 2.5 to 20 µg/mL, respectively. But, the highest antimicrobial activity against P. aeruginosa ATCC 27853were demonstrated by the extract of T. aestivum cv. Sultan 95 which grown in sea water and pre-treatment of seeds with acetyl salicylic acid.

Keywords


antimicrobial activity, Triticum aestivum cv. Tosunbey, T. aestivum cv. Sultan 95, salt stress

Full Text:

PDF

References


Andersson, A.A.M., Andersson, R., Piironen, V., Lampi, A.M., Nystrom, L., Boros, D., Fras, A., Gebruers, K., Courtin, C.M., Delcour, J.A., Rakszegi, M., Bedo, Z., Ward, J.L., Shewry, P.R., Aman, P. 2013: Contents of dietary fibre components and their relation to associated bioactive components in whole grain wheat samples from the healthgrain diversity screen. Food Chemistry, 136: 1243-1248.

Ashok, S.A. 2011: Phytochemical and pharmacological screening of wheat grass Juice (Triticum aestivum L.). International Journal of Pharmaceutical Sciences Review and Research, 9: 159-164.

Bandow, J.E., Brötz, H., Leichert, L.I., Labischinski, H., Hecker, M. 2003: Proteomic approach to understanding antibiotic action. Antimicrob Agents Chemother, 47: 948-55.

CLSI 2006: Clinical and Laboratory Standarts Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Seventh Edition. M07- A7

Das, A,, Raychaudhuri, U,, Chakraborty, R. 2012: Antimicrobial effect of edible plant extract on the growth of some foodborne bacteria including pathogens. Nutrafoods, 12: 83-88.

Foyer, C.H, Lelendais, M., Kunert, K.J. 1994: Photooxidative stress in plants. Physiologia Plantarum, 92: 696-717.

Gil, A., De La Fuente, E.B., Lenardis, A.E., Loopez Pereira, M., Suaorez, S.A., Bandoni, A., Van Baren, C., Di Leo Lira, P., Ghersa, C.M. 2002: Coriander essential oil composition from two genotypes grown in different environmental conditions. Journal of Agricultural Food Chemistry, 50: 2870-2877.

Huang, T., Xu, M., Lee, A., Cho, S., Qi, L. 2015: Consumption of whole grains and cereal fiber and total and cause-specific mortality: prospective analysis of 367,442 individuals. BMC Medicine, 13:59

Karakas, F.P., Turker, A.U. 2013: An efficient in vitro regeneration system for Bellis perennis L. and comparison of phenolic contents of field-grown and in vitro-grown leaves by LCMS/MS. Industrial Crops and Products. 48: 162-170

Kate, V.V. 2008: Physiological and biochemical studies in some medicinal plants: Tribulus terrestris L. and Pedalium murex L. Ph. D. Thesis submitted to Shivaji University, Kolhapur, Maharashtra, India.

Kotagiri, D., Shaik, K.B., Kolluru, V.C. 2017: Secondary metabolites and the antimicrobial potential of five different Coleus species in response to salinity stress. BioRxiv Plant Bio https://doi.org/10.1101/220368

Pallavi, K., Kumarswammy, G., Shruthi. B. 2011: Pharmacognostic investigation and antibacterial activity of Triticum aestivum. Journal of Pharmacy Research, 4: 3355-3359.

Paul, D., Lade, H. 2014: Plant-growth-promoting rhizobacteria to improve crop growth in saline soils: a review. Agron Sustain Dev, 34: 737-752.

Rojas, A., Hernandez, L., Pereda-Miranda, R., Mata, R. 1992: Screening for antimicrobial activity of crude drug extracts and pure natural products from Mexican medicinal plants. Journal of Ethnopharmacology, 35: 275-83

Saha, S., Islam, Z., Islam, S., Hossain, S., Islam, S.M.S. 2018: Evaluation of antimicrobial activity of wheat (Triticum aestivum L.) against four bacterial strains. SKUAST Journal of Research, 20 (1): 58-62.

Sultana, B., Anwar, F., Przybylski, R. 2007: Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. Trees Food Chemistry, 104: 1106–1114.

Sundaresan, A., Selvi, A., Manonmani, H.K. 2015: The anti-microbial properties of Triticum aestivum (Wheat Grass). International Journal of Biotechnology for Wellness Industries, 4: (3): 84-91.

Teanpaisan, R., Kawsud, P., Pahumunto, N., Puripattanavong, J. 2017: Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms. J Tradit Complement Med. 7(2): 172–177.

Türkoğlu, M., Baba, A., Özcan, H. 2006: Determination and evaluation of some physicochemical parameters in the Dardanelles (Canakkale Strait, Turkey) using multiple probe system and geographic information system. Hydrology Research, 37(3): 293-301.

Sewelam N, Kemel K, Peer MS. 2016: Global plant stress signalling: Reactive oxygen species at the cross-road. Frontiers of Plant Science. 7, 187.

Steward F.C. 1983: Plant Physiology, Academic Press, New York, 797p.

Vida, G., Szunics, L., Veisz, O., Bedo, Z., Lang, L., Arendas, T., Bonis, P., Rakszegi, M. 2014: Effect of genotypic, meteorological and agronomic factors on the gluten index of winter durum wheat. Euphytica, 197:61-71

Vimal, S.R., Gupta, J., Singh, J.S. 2018: Effect of salt tolerant Bacillus sp. and Pseudomonas sp. onwheat (Triticum aestivum L.) growth under soil: A comparative study Microbiology Research, 9(7462): 26–32.

Westh, H., Zinn, C.S., Rosdahl, V.T. 2004: An international multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries. Microbial Drug Resistance, 10: 169-76.


Refbacks

  • There are currently no refbacks.