Essential oil analysis of Dracocephalum moldavica L. from Serbia

Milica G Acimovic, Jovana Stanković, Mirjana Cvetković, Marina Todosijević, Milica Rat


Dracocephalum moldavica L., also called Moldavian balm or Moldavian dragonhead, is native to temperate climate of Asia, but it was naturalized in Eastern and Central Europe, North Africa, China and north-eastern United States. This is an annual plant, with numerous stems (up to 6), 22-45 cm high, and blue flowers arranged in pseudo-whorls growing in leaf axils. Essential oil accumulates in exogenous oil-containing cells at the dorsal sides of the leaves, and in the inflorescence. Because of this, the entire plant has a citrus-like flavor, resembling that of lemon balm and catnip. This plant is extensively used as a spice and for composition of tea blends, in food aromatization (canned fish, jams, candies, syrups), perfumery, alcohol industry, soaps and detergents. D. moldavica from Serbia contains geranial (29.6%), geranyl acetate (27.2%) and neral (19.4%) as the most abundant compounds. Further investigations will be focused on the influence of weather conditions on essential oil composition, as well as on bioactive potential of this essential oil.


Moldavian balm, Moldavian dragonhead, GC-MS analysis, geranial, geranyl acetate, neral

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Aćimović, M., Sikora, V., Brdar-Jokanović, M., Kiprovski, B., Popović, V., Koren, A., Puvača, N. 2019: Dracocephalum moldovica: cultivation, chemical composition and biological activity. Journal of Agronomy, Technology and Engineering Management, 2(1):153-167.

Ahl, H.A.H.S.A., Sabra, A.S., Gendy, A.N.G.E., Aziz, E.E., Tkachenko, K.G. 2015: Changes in content and chemical composition of Dracocephalum moldovica L. essential oil at different harvest dates. Journal of Medicinal Plants Studies, 3(2):61-64.

Amirnia, R., Manesh, M.F., Danesh, Y.R., Najafi, S., Seyyedi, N., Ghiyasi, M. 2017: Karyological study on Teheran ecotype of Moldavian balm (Dracocephalum moldavica L.). Journal of Molecular Biology and Biotechnology, 1(1):29-31.

Aprotosoaie, A.C., Mihai, C.T., Vochita, G., Rotinberg, P., Trifan, A., Luca, S.V., Petreus, T., Gille, E., Miron, A. 2016: Antigentoxic and antioxidant activities of polyphenolic extract from European Dracocephalum moldavica L. Industrial Crops and Products, 79:248-257.

Aslanipour, B., Heidari, R., Farnad, N. 2017: Phenolic combination and comparison of antioxidant activity in three different alcoholic extracts of Dracocephalum moldavica L. Turkish Journal of Agriculture - Food Science and Technology, 5(3):199-206.

Aziz, E.E., Hussein, M.S., Wahba, H.E., Razin, A.M. 2013: Essential oil constituents of Dracocephalum moldavica L. grown under salt stress and different sources of soil amendment. Middle-East Journal of Scientific Research, 16(5):706-713.

Chu, S.S., Liu, S.L., Liu, Q.Z., Liu, Z.L., Du, S.S. 2011: Composition and toxicity of Chinese Dracocephalum moldavica (Labiatae) essential oil against two grain storage insects. Journal of Medicinal Plants Research, 5(18):4621-4626.

Ding, S.E., Cixi, T., Charles, P. 2015: Synthesis and toxicity of Chinese Dracocephalum moldavica (Labiatae) fundamental oil against two grain storage insect. African Journal of Insects, 2(2):77-81.

Ehsani, A., Alizadeh, O., Hashemi, M., Afshari, A., Aminzare, M. 2017: Phytochemical, antioxidant and antibacterial properties of Melissa officinalis and Dracocephalum moldavica essential oil. Veterinary Research Forum, 8(3):223-229.

El-Baky, H.H., El-Bsroty, G.S. 2008: Chemical and biological evaluation of the essential oil of Egyptian moldavian balm (Dracocephalum moldavica L.). International Journal of Integrative Biology, 3(3):202-208.

Eshan, K.A., Bial, G., Kiarash, A.P.R., Mesut, U. 2014: GC/MS analysis of bioactive components of Dracocephalum moldavica L., treated by boric acid doses. Tarum Bilimleri Arastirma Dergisi, 7(1):19-21.

Fallah, S., Rosttaei, M., Lorigooini, Z., Surki, A.A. 2018: Chemical compositions of essential oil and antioxidant activity of dragonhead (Dracocephalum moldavica) in sole crop and dragonhead-soybean (Glycine max) intercropping system under organic manure and chemical fertilizers. Industrial Crops and Products, 115:158-165.

Golparvar, A.R., Hadipanah, A., Gheisari, M.M., Khaliliazar, R. 2016: Chemical constituents of essential oil of Dracocephalum moldavica L. and Dracocephalum kotschyi Boiss. from Iran. Acta Agriculturae Slovenica, 107:25-31.

Hegazy, M.H., Alzuaibr, F.M.A., Mahmoud, A.A., Mohamed, H.F.Y., Said-Al Ahl, H.A.H. 2016: The effects of zinc application and cutting on growth, herb, essential oil and flavonoids in three medicinal Lamiaceae plants. European Journal of Medicinal Plants 12(3):1-12.

Hussein, M.S., El-Sherbeny, S.E., Khalil, M.Y., Naguib, N.Y., Aly, S.M. 2006: Growth characters and chemical constituents of Dracocephalum moldavica L. plants in relation to compost fertilizer and planting distance. Scientia Horticulturae, 108:322-331.

Janmohammadi, M., Nouraein, M., Sabaghnia, N. 2017: Influence of different weed management technique on the growth and essential oils of dragonhead (Dracocephalum moldavica L.). Romanian Biotechnological Letters, 22(5):12950-12960.

Keikhaie, K.R., Jahantigh, H.R., Bagheri, R., Kehkhaie, A.R. 2018: The effects of the ethanol extract of Dracocephalum moldavica (Badrashbu) against strains of antibiotic-resistant Escherichia coli and Klebsiella pneumonia. International Journal of Infection, 5(1):e65295.

Kišgeci, J., Adamović, D., Janjatović, V., Mijavec, A. 1982: Introduction of medicinal and aromatic plant species in Vojvodina Province. Bilten za hmelj, sirak i lekovito bilje, 39(1):5-24.

Kotyuk, L.A., Rakhmetov, D.B. 2017: Component composition of essential oil from Dracocephalum moldavica L. grown in the Ukrainian polissya. International Scientific Symposium “Conservation of Plant Diversity”, 1-3, June, 2017, Chisinau, Moldova.

Maham, M., Akbari, H., Delazar, A. 2013: Chemical composition and antinociceptive effect of the essential oil of Dracocephalum moldavica L. Pharmaceutical Sciences, 18(4):187-192.

Maimaitiyiming, D., Hu, G., Aikemu, A., Hui, S.W., Zhang, X. 2014: The treatment of Uygur medicine Dracocephalum moldavica L on chronic mountain sickness rat model. Pharmacognosy Magazine, 10(40):477-482.

Martínez-Vázquez, M., Estrada-Reyes, R., Martínez-Laurrabaquio, A., López-Rubalcava, C., Heinze, G. 2012: Neuropharmacological study of Dracocephalum moldavica L. (Lamiaceae) in mice: Sedative effect and chemical analysis of an aqueous extract. Journal of Ethnopharmacology, 141:908-917.

Naderifar, M., Sonboli, A., Gholipour, A. 2015: Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance. Bangladesh Journal of Plant Taxonomy, 22(2):99-110.

Najafi, M., Ghasemian, E., Fathiazad, F., Garjani, A. 2009: Effects of total extract of Dracocephalum moldavica on ischemia/reperfusion induced arrhythmias and infarct size in the isolated rat heart. Iranian Journal of Basic Medical Sciences, 11(4):229-235.

Omidbaigi, R., Yavari, S., Hassani, M.E., Yavari, S. 2010: Introduction of autoploidy in dragonhead (Dracocephalum moldavica L.) by colchicines treatment. Journal of Fruit and Ornamental Plant Research, 18(1):23-35.

Oniszczuk, T., Wójtowicz, A., Kocira, S., Żelizko, K., Oniszczuk, A., Dib, A. 2017: The use of Moldavian dragonhead bagasse waste in extruded products. IX International Scientific Symposium "Farm Machinery and Processes Management in Sustainable Agriculture", Lublin, Poland.

Pak, Z.H., Abbaspour, H., Karimi, N., Fattahi, A. 2016: Eco-friendly synthesis and antimicrobial activity of silver nanoparticles using Dracocephalum moldavica seed extract. Applied Sciences, 6:69.

Sun, Y., Liu, T., Dai, X., Jiang, Z., Gao, Z., Zhang, M., Wang, D., Zheng, Q. 2014: Neuroprotective effect of Dracocephalum moldavica L. total flavonoids in transient cerebral ischemia in rats. Annual Research and Review in Biology, 4(12):1915-1926.

Weremczuk-Jeżyna, I., Grzegorczyk-Karolak, I., Frydrych, B., Hnatuszko-Konka, K., Gerszberg, A., Wysokińska, H. 2017: Rosmarinic acid accumulation and antioxidant potential of Dracocephalum moldavica L. cell suspension culture. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1):215-219.

Wojtowicz, A., Oniszczuk, A., Oniszczuk, T., Kocira, S., Wojtunik, K., Mitrus M., Kocira A., Widelski, J., Skalicka-Wozniak, K. 2017: Application of Moldavian dragonhead (Dracocephalum moldavica L.) leaves addition as a functional component of nutritionally valuable corn snacks. Journal of Food Science and Technology, 54(10):3218-3229.

Zeng, C., Jiang, W., Yang, X., He, C., Wang, W., Xing, J. 2018: Pretreatment with total flavonoid extract from Dracocephalum Moldavica L. attenuates ischemia reperfusion-induced apoptosis. Scientific Reports, 8:17491.


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