Serbian Rutaceae species: comparison of chemical profiles and radical scavenging activity

Dragana R. Pavlović, Bojan Zlatković, Slavoljub Živanović, Dušanka Kitić, Tatjana Golubović

Abstract


Family Rutaceae is presented in Serbian flora with 3 genera and 4 species: Ruta graveolens L., Dictamnus albus L., Haplophyllum suaveolens (DC.) G. Don and H. boissieranum Vis. & Pančić. Comparative study of the phytochemical variability and antioxidant activity of ethanolic and methanolic extracts of areal parts of this species was performed. Total flavonoid content was determined spectrophotometricaly and HPLC method was employed for quantification of coumarins. Fast antioxidant screening of samples was done in DPPH test. Flavonoid content of the tested samples varied from 2.82±0.27 to 27.72±0.15 mg of rutin equivalent (Ru)/g of extract. Psolaren, bergapten and xantotoxin were detected and quantified only in R. graveolens extracts. All the tested samples exhibited notable potential in DPPH radicals scavenging assay. Yet, considerably lower activity was measured for Haplophyllum extracts. The present study brings forward the new data regarding the chemical profiles and antiradical activities of named Rutaceae species.


Keywords


Ruta; Dictamnus; Haplophyllum; flavonoids; HPLC of coumarins; DPPH assay

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References


Asgarpanah J., Khoshkam R. 2012: Phytochemical and pharmacological properties of Ruta graveolens L. Journal of Medicinal Plants Research 6(23): 3942-3949.

Beis R., Öztürk Y., Aydin S., Gürer F. 2005: Pharmacological and toxicological effects of gas plant (Dictamnus albus L.). Turkish Journal of Pharmaceutical Sciences 2 (3): 111-124.

Cuendet M., Hostettmann K., Potterat O. 1997: Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta 80: 1144-1152.

Diklić N., 1973: Rutaceae In: Josifović M, Stjepanović L., Janković M.M., Gajić M., Kojić M., Diklić N. (eds.), Flora SR Srbije V. Srpska akademija nauka i umetnosti, Beograd.

Council of Europe, 2017: European Pharmacopeia 9.0. 9th Edition Council of Europe, Strasbourg.

Ivanova A, Mikhova B, Stambolijska T, Kostova I. 2001: Lignan and coumarin glycosides from Haplophyllum suaveolens. Z Naturforsch C 56(5-6): 329-33.

Jančić R. 2002: Botanika farmaceutika. Službeni list SRJ, Beograd.

Lamaison J.L., Carnat A. 1990: Teneurs en principaux flavonoids des fleurs de Crataegeus monogyna Jacq et de Crataegeus laevigata (Poiret D. C.) en fonction de la vegetation. Pharmaceutica Acta Helvetica 65, 315-320.

Mabry T.J., Markham K. R., Thomas M. B. 1970: The Systematic Identification of Flavonoids. Springer-Verlag, Berlin.

Melougha M.M., Chob E., Chuna O.K. 2018: Furocoumarins: A review of biochemical activities, dietary sources and intake, and potential health risks. Food and Chemical Toxicology 113: 99–107.

Michael J.P. 2003: Quinoline, quinazoline and acridone alkaloids. Natural Products Reports 20(5): 476-93.

Middleton E.J. 1984: The flavonoids. Trends in Pharmacological Science, 8:335-338.

Mihajilov-Krstev T., Zlatković B., Ilić M., Stankov-Jovanović V., Mitić V. 2015: Antimicrobial and antioxidant potential of wild growing Silene baccifera (L.) Roth. (Caryophyllaceae) fruits juice. Biologica Nyssana 6 (2): 55-58.

Pavlović D.R., Branković S., Kovačević N., Kitić D., Veljković S. 2011: Comparative Study of Spasmolytic Properties, Antioxidant Activity and Phenolic Content of Arbutus unedo from Montenegro and Greece. Phytotherapy Research 25(5): 749-54.

Pavlović D.R., Tasić-Kostov M., Marčetić M., Lakušić B., Kitić D., Savić S., Kovačević N. 2013: Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts. Rivista Italiana Delle Sostanze Grasse 90 (4): 255-264.

Pavlović D.R., Vukelić M., Najman S., Kostić M., Zlatković B., Mihajilov-Krstev T., Kitić D. 2014: Assessment of polyphenol content, in vitro antioxidant, antimicrobial and toxic potentials of wild growing and cultured rue. Journal of Applied Botany and Food Quality 87: 175 – 181.

PDR, 2000: PDR for Herbal Remedies. 2nd Edition, Thomson PDR, Medical Economics Company, Montvale, New Jersey.

Stojković D., Jančić R., Lakušić B., Slavkovska V. 2007: Samonikle lekovite biljke Stare planine priručnik za održivo sakupljanje. Svetska unija za zaštitu prirode Zavod za zaštitu prirode Srbije, Beograd.

Tasić S., Šavikin Fodulović K., Menković N. 2004: Vodič kroz svet lekovitog bilja. Valjevac, Valjevo.

Tucakov J., 1997: Lečenje biljem. VII Edition, Rad, Beograd.

Tutin T.G., 1968: Rutaceae In: Tutin T.G., Heywood V.H., Burges N.A., Moore D.A., Valentine D.H., Walters S.M., Webb D.A. (eds.), Flora Europaea 2. University Press, Cambridge.

Veselinović J.B., Matejić J.S., Veselinović A.M., Sokolović D. 2016: Coumarin structure as a lead scaffold for antibacterial agents - molecular docking study. Biologica Nyssana 7(2): 167-170.

Wrześniok D., Beberok A., Rok J., Delijewski M., Hechmann A., Oprzondek M., Rzepka Z., Bacler-Żbikowska B., Buszman E. 2017: UVA radiation augments cytotoxic activity of psoralens in melanoma cells. International Journal of Radiation Biology 93(7): 734-739.

Yang R.-Y., Tsou S.C.S., Lee T.-C., Wu W.-J., Hanson P.M., Kuo G., Engle L.M., Lai P.-Y. 2006: Distribution of 127 edible plant species for antioxidant activities by two assays. Journal of Science and Food Agriculture 86: 2395-2403.


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