Genetic diversity of the critically endangered Verbascum davidoffii Murb. (Scrophulariaceae) and implications for conservation
Abstract
Verbascum davidoffii Murb. (Scrophulariaceae), one of the rarest plant species in Bulgarian flora, is a local endemic, protected by the National Biodiversity Act, included in the Red List of vascular plants, as well as in the Red Data Book of Bulgaria with conservation status “Critically Endangered”. Its distribution is limited due to anthropogenic pressure, specific ecological requirements and low reproductive capability. In this study, we aimed to measure the genetic diversity level in the unique single world population of Verbascum davidoffii located in Pirin National Park, Bulgaria. We found high genetic diversity in the excitant population of the species. The present study indicates that the primary objective in conservation of Verbascum davidoffii is to preserve as much as possible of its.
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Assyov, B., Denchev, C. 2015: Verbascum davidoffii Murb. In: Biserkov, V. et al. (eds.), Digital edition of Red Data Book of Republic of Bulgaria. BAS & MOEW, Sofia. http://e-ecodb.bas.bg/rdb/en/vol1/Verdavid.html.
Biserkov, V. et al. (eds.) 2015: Digital edition of Red Data Book of Republic of Bulgaria. BAS & MOEW, Sofia. http://e-ecodb.bas.bg/rdb/en/.
Camacho, F.J., Liston, A. 2001: Population structure and genetic diversity of Botrychium pumicola (Ophioglossaceae) based on inter-simple sequence repeats (ISSR). American Journal of Botany, 88 (6): 1065-1070.
Doyle, J.J., Doyle, J.L. 1987: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
Fang, D.Q., Roose, M.L. 1997: Identification of closely related citrus cultivars with inter-simple sequence repeat genetics. Theoretical and Applied Genetics, 95 (3): 408-417.
Firat, M. 2015: Verbascum kurdistanicum (Scrophulariaceae), a new species from Hakkari, Turkey. PhytoKeys, 52: 89-94.
Gonzalo-Turpin H., Hazard, L. 2009: Local adaptation occurs along altitudinal gradient despite the existence of gene flow in the alpine plant species Festuca eskia. Journal of Ecology, 97 (4): 742-751.
Hamrick, J.L., Godt, M.J.W. 1989: Allozyme diversity in plant species. In: Brown, A.H.D., Clegg, M.T., Kahler, A.L., Weir, B.S. (eds.), Plant population genetics, breeding and genetic resources. Sunderland, MA: Sinauer Associates, pp. 43-63.
Helenurm, K. 2001: High levels of genetic polymorphism in the insular endemic herb Jepsonia malvifolia. Journal of Heredity, 92 (5): 427-432.
Huang, Y., Zhang, C.Q., Li, D.Z. 2009: Low genetic diversity and high genetic differentiation in the critically endangered Omphalogramma souliei (Primulaceae): implications for its conservation. Journal of Systematics and Evolution, 47 (2): 103–109.
Idrees, M., Irshad, M. 2014: Molecular markers in plants for analysis of genetic diversity: a review. European Academic Research, 2 (1): 1513-1540.
Jia, J., Zeng, L., Gong, X. 2016: High genetic diversity and population differentiation in the critically endangered plant species Trailliaedoxa gracilis (Rubiaceae). Plant Molecular Biology Reporter, 34 (1): 327-338.
Li, Y.Y., Guan, S.M., Yang S.Z., Luo, Y., Chen, X.Y. 2012: Genetic decline and inbreeding depression in an extremely rare tree. Conservation Genetics, 13 (2): 343-347.
Nybom, H. 2004: Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13 (5): 1143-1155.
Peakall, R., Smouse, P.E. 2012: GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics, 28 (19): 2537-2539.
Petrova, G., Dzhambazova, T., Moyankova, D., Georgieva, D., Michova, A., Djilianov, D., Moeller, M. 2014: Morphological variation, genetic diversity and genome size of critically endangered Haberlea (Gesneriaceae) populations in Bulgaria do not support the recognition of two different species. Plant Systematics and Evolution, 300 (1): 29-41.
Rao, V.R., Hodgkin, T. 2002: Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell, Tissue Organ Culture, 68 (1): 1-19.
Stefanova-Gateva, B. 1995: Verbascum L. In: Kožuharov, S. (ed.), Flora Reipublicae Popularis Bulgaricae 10: 26-100, Acad. "Prof. M. Drinov", Serdicae, Sofia, Bulgaria (in Bulgarian).
Turchetto, C., Segatto, A.L.A., Mäder, G., Rodrigues, D.M., Bonatto, S.L., Freitas, L.B. 2016: High levels of genetic diversity and population structure in an endemic and rare species: implications for conservation. AoB PLANTS 8: plw002; doi:10.1093/aobpla/plw002.
Walther, G.-R., Beißner, S., Burga, C.A. 2005: Trends in the upward shift of alpine plants. Journal of Vegetation Science, 16 (5): 541-548.
Willi, Y., Van Buskirk, J., Hoffmann, A.A. 2006: Limits to the adaptive potential of small populations. Annual Review of Ecology, Evolution and Systematics, 37: 433-458.
Wolfe, A.D., Liston, A. 1998: Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis, P.S., Soltis, D.E., Doyle, J.J. (eds.), Molecular systematics of plants: DNA sequencing. Kluwer, New York, pp. 43-86.
Xue, D.-W., Ge, X.-J., Hao, G., Zhang, C.-Q. 2004: High genetic diversity in a rare, narrowlu endemic primrose species: Primula interjacens by ISSR analysis. Acta Botanica Sinica, 46 (10): 1163-1169.
Zawko, G., Krauss, S.L., Dixon, K.W., Sivasithamparam, K. 2001: Conservation genetics of the rare and endangered Leucopogon obtectus (Ericaceae). Molecular Ecology, 10 (10): 2389-2396.
Zhu, Y., Geng, Y., Tersing, T., Liu, N., Wang, Q., Zhong, Y. 2009: High genetic differentiation and low genetic diversity in Incarvillea younghusbandii, an endemic plant of Qinghai-Tibetan Plateau, revealed by AFLP markers. Biochemical Systematics and Ecology 37 (5): 589-596.
Zietkiewicz, E., Rafalski, A., Labuda, D. 1994: Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20 (2): 176-183.
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