Oxidative stress in bromus seedlings treated with Salvia sclarea L. aqueous extract

Jovana Šućur, Dejan Prvulović, Goran Anačkov, Đorđe Malenčić


Extensive use of synthetic pesticides has negative effects on the environment and on human and animal health. Knowledge on allelopathic interactions could provide effective tools for a better exploitation of natural resources in the management of weeds without using herbicides. One of highly resistant weed species is bromus. The effects of two concentrations (0.1% and 0.2%) of Salvia sclarea L. aqueous extract on the activity of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) in leaves and roots of bromus (Bromus mollis L.) seedlings, were examined. Our results showed that both concentrations of the extract used (0.1% and 0.2%) stimulated the significant increase of the superoxide dismutase activity in leaves and roots of bromus 72 hours and 120 hours after the treatment. The significant increase of the catalase activity was recorded in roots of bromus 72 h after the treatment. Two tested extract concentrations affected activity of the antioxidant enzymes in the same way, but the higher activity was observed in the roots treated with higher concentration (0.2%). The increase of the activities of antioxidant enzymes, in response to stress induced by S. sclarea aqueous extract, indicate that the plant extract possesses allelopathic activity on treated plant.


Allelopathy, Biopesticides, Bromus mollis L., Salvia sclarea L.

Full Text:



Fakoorziba, M.R., Moemenbellah–Fard, M.D., Azizi, K., Shekarpoor, H., Alipoor, H. 2014: Excito–Repellency Effects of Salvia sclarea L. (Lamiaceae) Extracts on Adult House Flies, Musca domestica L. (Diptera: Muscidae). Journal of Health Sciences and Surveillance System, 2(1): 1–7.

Gella, D., Ashagre, H., Negewo, T. 2013: Allelopathic effect of aqueous extracts of major weed species plant parts on germination and growth of wheat. Journal of Agricultural and Crop Research, 1(3): 30–35.

Hudaib, M., Grazia Bellardi, M., Rubies–Autonell,C., Fiori, J., Cavrini, V. 2001: Chromatographic (GC–MS, HPLC) and virological evaluations of Salvia sclarea infected by BBWV-I. Il Farmaco, 56: 219–227.

Hussain, M.I., Reigosa, M.J. 2011: Allelochemical stress inhibits growth, leaf water relations, PSII photochemistry, non–photochemical fluorescence quenching, and heat energy dissipation in three C3 perennial species. Journal of Experimental Botany, doi:10.1093/jxb/err161.

Nasermoadeli, S., Rowshan, V. 2013: Comparison of Salvia sclarea L. Essential Oil Components in Wild and Field Population. International Journal of Agriculture and Crop Sciences, 5(8): 828–831.

Omezzine, F., Bouaziz, M., Simmonds, M.S.J., Haouala, R. 2014: Variation in chemical composition and allelopathic potential of mixoploid Trigonella foenum–graecum L. with developmental stages. Food Chemistry, 148: 188–195.

Sharma, M., Satsangi, G.P. 2013: Potential Allelopathic Influence of Sunflower (Helianthus Annuus L.) on Germination and Growth behavior of Two Weeds in–vitro Condition. International Journal of Biotechnology and Bioengineering Research, 4(5):421–426.

Soltys, D., Krasuska, U., Bogatek, R., Gniazdowska, A. 2013: Allelochemicals as Bioherbicides–Present and Perspectives, Herbicides–Current Research and Case Studies in Use, doi: 10.5772/56185.

Weir, T.L., Park, S., Vivanco, J.M. 2004: Biochemical and physiological mechanisms mediated by allelochemicals. Current Opinion in Plant Biology, 7: 472–479.

Xuan, T.D., Shinkichi, T., Khanh, D.T., Min, C.I. 2005: Biological control of weeds and plant pathogens in paddy rice by exploiting plant allelopathy: an overview. Crop Protection, 24: 197–206.


  • There are currently no refbacks.