Micropropagation of Micromeria juliana (L.) Benth. ex Rchb. (Lamiaceae)

Svetlana Tošić, Sanja Nikolić, Marija Jovanović, Bojan Zlatković, Dragana Stojičić


Micromeria juliana belongs to family Lamiaceae, whose representatives are characterized by a significant level of essential oils and antioxidant components. Several species of genus Micromeria contain biologically active substances and are therefore used in folk medicine, food industry and cosmetic industry. Methods of their tissue culture may provide rapid mass multiplication of plants for various purposes, including research on production, accumulation and metabolism of important secondary metabolites. The goal of this paper was to determine the protocol for regeneration of M. juliana plants through use of nodal explants on nutritive substrate with various growth regulators. The greatest number of axillary buds was formed in explants grown on MS nutritive medium with 3 μM benzyladenine (BA) and 0.57 μM indole-3-acetic acid (IAA). The explants grown at the medium without any growth regulators and the medium with auxin have shown spontaneous root formation.

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Abou-Jawdah, Y., Wardan, R., Sobh, H., Salameh, A. 2004: Antifungal activities of extracts from selected Lebanese wild plants against plant pathogenic fungi. Phytopathologia Mediterranea, 43: 377–386.

Aslan, I., Calmasur, O., Sahon, F., Caglar, O. 2005: Insecticidal effects of essential plant oils against Ephestia kuehniella (Zell.), Lasioderma serricorne (F.) and Sitophilus granarius (L.). Journal of Plant Diseases and Protection, 112 (3): 257–267.

Bezić, N., Dunkić, V., Vuko, E. 2013: Antiphytoviral Activity of Essential Oils of Some Lamiaceae Species and There Most Important Compounds on CMV and TMV. In: Méndez-Vilas, A. (ed.), Microbial pathogens and strategies for combating them: science, technology and education.2: 982-988.Formatex Research Center, Spain.

Chater, A.O., Guinea E. 1973: Micromeria Bentham. In:Tutin, T.G., J.R., Heywood, V.H., Moore, Valentine, S.M., Webb, D.A. (eds.), Flora Europaea. 3:167-170. The University Press, Cambridge.

Diaz, M.S., Palacio, L., Figueroa, A.C., Goleniowski, M.E. 2012: In Vitro Propagation of Muna-Muna (Clinopodium odorum (Griseb.) Harley). Biotechnology Research International, 2012: 1-6.

Diklić, N. 1974: Micromeria Benth. In: Josifović, M. (ed.), Flora Srbije 6:458-462. Srpska akademija nauka i umetnosti, Beograd.

Dode, L.B., Seixas, F.K., Schuch, M.W., Braga, E.J.B., Bobrowski, V.L. 2003: In vitro clonal propagation of Ocimum basilicum L. (Lamiaceae). Acta Scientiarum. Biological Sciences, 25 (2): 439-441.

Doroszenko, A. 1986: Taxonomic Studies on the Satureja Complex (Labiatae). Ph.D. Thesis, University of Edinburgh, Edinburgh (manuscr.).

Dudai, N., Poljakoff-Mayber, A., Mayer, A.M., Putievsky, E., Lerner, H.R. 1999: Essential oils as allelochemicals and their potential use as bioherbicides. Journal of Chemical Ecology, 25(5): 1079-1089.

Dudai, N., Chaimovitsh, D., Larkov, O., Fischer, R., Blaicher, Y., Mayer, A.M. 2009: Allelochemicals released by leaf residues of Micromeria fruticosa in soils, their uptake and metabolism by inhibited wheat seed. Plant Soil, 314: 311–317.

Duru, M.E., Öztürk, M., Ugur, A., Ceylan, O. 2004: The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey. Journal of Ethnopharmacology, 94: 43–48.

Fay, M.F. 1992: Conservation of rare and endangered plants using in vitro methods. In Vitro Cellular and Developmental Biology. Plant, 28: l-4.

George, E.F., Sherrington, P.D. 1984: Plant propagation by tissue culture. Exgetics Ltd. Basingstoke, England.

Ghanti, K., Kaviraj, C.P., Venugopal, R.B., Jabeen, F.T.Z., Rao, S. 2004: Rapid regeneration of Mentha piperita L. from shoot tip and nodal explants. Indian Journal of Biotechnology, 3: 594-598.

Güllüce, M., Sökmen, M., Şahin, F., Sökmen, A., Adigüzel, A., Özer, H. 2004: Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L.) Druce ssp. serpyllifolia (Bieb.) P.H. Davis plants from the eastern Anatolia region of Turkey. Journal of the Science of Food and Agriculture, 84(7): 735–741.

Janarthanam, B., Sumathi, E. 2012: Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.. Bangladesh Journal of Scientific and Industrial Research, 47: 433 – 436.

Marinković, B., Marin, P.D., Knežević-Vukčević, J., Soković, M.D., Brkić, D. 2002: Activity of essential oils of thee Micromeria spesies (Lamiaceae) againd micromycetes and bacteria. Phytoterapy Research, 16 (4): 336-339.

Martinez-Pulido, C., Harry, I.S., Thorpe, T.A. 1992: Optimization of bud induction in cotyledonary explants of Pinus canariensis. Plant Cell Tissue and Organ Culture, 29:247–255.

Misic, D., Grubisic, D., Konjevic, R. 2006: Micropropagation of Salvia brachyodon through nodal explants. Biologia Plantarum, 50 (3): 473-476.

Molia, M.F.A., Kwapata, M.B. 2000: Apomictic embryo development and survival in Uapaca kirkiana under in vitro and in vivo seed germination. Scientia Horticulturae, 83: 139–147.

Murashige, T., Skoog, F. 1962: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-497.

Nirmal, K.S., Sehgal, C.B. 1999: Micropropagation of „Holy Basil‟ (Ocimum sanctum Linn.) from young inflorescences of mature plants. Plant Growth Regulation, 29: 161-166.

Özcan, M. 1999: Antifungal effects of Micromeria myrtifolia Boiss and Hohen in Boiss. and Prangos uechtritzii (Boiss.) Hawsskn decoctions. Acta Alimentaria, 28: 355–360

Rahman, M.M., Ankhi, U.R., Biswas, A. 2013: Micropropagation of Mentha viridis L.: An aromatic medicinal plant. International Journal of Pharmacy and Life Sciences, 4 (9): 2926-2930.

Reed, B.M., Sarasan, V., Kane, M., Bunn, E., Pence, V.C. 2011: Biodiversity conservation and conservation biotechnology tools. In Vitro Cellular and Developmental Bioogy. Plant, 47: 1–4.

Saha, S., Kader, A., Sengupta, C., Ghosh, P. 2012: In Vitro Propagation of Ocimum gratissimum L. (Lamiaceae) and Its Evaluation of Genetic Fidelity Using RAPD Marker. American Journal of Plant Sciences, 3: 64-74.

Sarac N., Ugur A. 2007: Antimicrobial activites and usage in folkloric medicine of some Lamiaceae species growing in Mugla Turkey. EurAsian Journal of BioSciences, 4: 28-37.

Šarić-Kundalić, B., Dobes, C., Klatte-Asselmeyer, V., Saukel, J. 2011: Ethnobotanical survey of traditionally used plants in human therapy of east, north and north-east Bosnia and Herzegovina. Journal of Ethnopharmacology, 133: 1051–1076.

Sharma, D.K., Sharma, T. 2013: Biotechnological approaches for biodiversity conservation. Indian Journal of Scientific Research, 4 (1): 183-186.

Šilić, Č. 1979: Monografija rodova Satureja L., Calamintha Miller, Micromeria Bentham, Acinos Miller i Clinopodium L. u flori Jugoslavije. Zemaljski Muzej, Sarajevo.

Stojanovic, G., Palic, I. 2008: Antimicrobial and Antioxidant Activity of Micromeria Bentham Species. Current Pharmaceutical Design, 14 (29): 3196-3202.

Tabanca, N., Kirimer, N., Demirci, B., Demirci, F., Baser, K.H.C. 2001: Composition and Antimicrobial Activity of the Essential Oils of Micromeria cristata subsp. phrygia and the Enantiomeric Distribution of Borneol. Journal of Agricultural and Food Chemistry, 49: 4300-4303.

Tavers, A.C., Pimenta, M.C., Goncalves, M.T. 1996: Micropropagation of Melissa officinalis through prokiferation af axillary shoots, Plant Cell Reports, 15: 441-444.

Tejavathi, D.H., Indira, M.N. 2011: In vitro regeneration of multiple shoots from the nodal explants of Drymaria cordata (L.) Wild. Ex. Roem. and Schult. The Bioscan, 6(4): 657-660.

Tošić, S., Stojičić, D., Stankov-Jovanović, V., Mitić, V., Mihajilov-Krstev, T., Zlatković, B. 2015: Chemical composition, antioxidant and antimicrobial activities of micropropagated and native Micromeria pulegium (Lamiaceae) extracts. Oxidation Communications, 38 (1): 55-66.

van Staden, E., Zazimalova, E., George, E.F. 2008: Plant growth regulators II: cytokinins, their analogues and antagonists. In: George, E.F., Hall, M.A., De Klerk, G.-J. (Eds.): Plant Propagation by Tissue Culture, (3rd edition). 1: 205–226. Springer, Bachground, Dordrecht, Netherlands.

Vladimir-Knežević, S., Blažeković, B., Bival Štefan, M., Alegro, A., Köszegy, T., Petrik, J. 2011: Antioxidant activities and polyphenolic contents of three selected Micromeria species from Croatia. Molecules, 16: 1454-1470.

Zielinska, S., Piatczak, E., Kalemba, D., Matkowski, A. 2011: Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fischer & C.A.Meyer) O. Kuntze. Plant Cell, Tissue and Organ Culture, 107: 161–167.


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