In-vitro & in-silico anticholinesterase, α-amylase and anti-urease inhibitory activities of Zygophyllum simplex L. ethanolic extract

Boumediene Bentameur, Imadeddine Kadi, Zeyneb Benramdane, Thamere Cheriet, Djamel Sarri, DOI: 10.46793/BiolNyss.17.1.11B

Abstract


In this study, we aimed to evaluate total phenolic and flavonoid contents (TPC & TFC), anti-AChE, anti-BChE, anti-urease and α-amylase activities of the ethanol extract (ZSE), obtained from the aerial parts of the Algerian Zygophyllum simplex L. Molecular docking was applied on the most abundant components; identified previously from the plant around the world, which are six phenolic compounds, eleven flavonoids and five ursane-type saponins. Moderate values were observed for both TPC (29.674 ± 2.395 mg gallic acid/g extract) and TFC (35.843 ± 0.836 mg Quercetin/g extract). ZSE displayed weak AChE (IC50: 148.50±1.09 µg/mL) and α-amylase (IC50: 101.63±0.61 µg/mL) inhibitory activity, while remarkable effects were observed for both anti-BChE (IC50: 42.09±3.88 µg/mL) and anti-urease (IC50: 63.61±1.29 µg/mL). In addition, molecular docking reveals human BChE (PDB ID 4EY4) and Bacillus pasteurii urease (PDB ID 4UBP), to be potential protein targets for Z. simplex components.

Keywords


Zygophyllum simplex, anticholinesterase, α-amylase, anti-urease, molecular docking

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References


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