Development of an in vitro propagation protocol for Lathyrus undulatus Boiss.: A threatened species endemic to Türkiye

Buse Çökmez, Arda Acemi, Fazıl Özen, DOI: 10.46793/BiolNyss.17.1.25C

Abstract


Lathyrus undulatus Boiss. is a threatened plant species endemic to Türkiye from the Fabaceae family. The current study is based on investigating the effects of several cytokinins and auxins on organ development to develop an in vitro propagation protocol for conservation purposes in the species. Nodal segments from adult individuals were cultured in 6-benzylaminopurine (BAP), thidiazuron (TDZ), and N6-(2-isopentenyl) adenosine (2IP), and generated in vitro shoots were cultured on full-, half-, and quarter-strength medium with 3-indoleacetic acid (IAA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA). The BAP treatments were more effective at inducing shoots from nodal segments, resulting in more nodes per shoot. TDZ treatments were superior to other cytokinins tested for shoot production from nodal segments, while 2IP treatments were superior for triggering shoot elongation. Rooting experiments demonstrated that L. undulatus is a problematic species to root in vitro. Neither full-strength nor reduced-strength culture medium induced rooting. Likewise, shoots were found to be unresponsive to IBA treatments. NAA treatments triggered a rooting response in full- and half-strength culture medium, while IAA induced a limited rooting response in half- and quarter-strength culture medium. In the rooting phase, NAA reached a maximum root-induction rate of only 20%, which should be studied further to increase the response and ensure enhanced integration into the soil for conservation purposes. Our findings indicated that while adenine-type cytokinins are superior at inducing shoot elongation, phenylurea-type cytokinins should be favored for multiple shoot induction in L. undulatus.

Keywords


Auxins; Cytokinins; Medium strength; Nodal segment; Sustainable biodiversity

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References


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