Polyphenols and antioxidant capacity of selected Compositae species

Nenad Zlatic, Danilo Stojanović, Milan Stanković, DOI: 10.46793/BiolNyss.16.1.24Z

Abstract


Species belonging to the Compositae family are known for their rich content of phenolic compounds and flavonoids with notable antioxidant properties. Given the increasing demand for natural antioxidants in various industries, this study aims to evaluate the total phenolic and flavonoid content alongside antioxidant activities of ethanolic extracts of six Compositae species; to identify interspecific differences and highlight their potential for use in pharmaceutical, cosmetic, and food sectors. The investigated species include Centaurea salonitana, C. atropurpurea, Achillea atrata, A. clavennae, Filago arvensis, and Xeranthemum annuum. Phenolic content was quantified utilizing the Folin–Ciocalteu method (GAE), while flavonoid content was measured as rutin equivalent (RuE). The antioxidant activity was determined via the DPPH assay (IC50 values). Significant interspecific variation was observed; notably, Achillea atrata exhibited the highest phenolic content, whereas Centaurea salonitana was distinguished by its elevated flavonoid content.


Keywords


antioxidant activity, Asteraceae, flavonoids, phenols, secondary metabolites

Full Text:

PDF

References


Aktumsek, A., Zengin, G., Guler, G. O., Cakmak, Y. S., Duran, A. (2011). Screening for in vitro antioxidant properties and fatty acid profiles of five Centaurea L. species from Turkey flora. Food and Chemical Toxicology, 49, 2914–2920. https://doi.org/10.1016/j.fct.2011.08.016

Aktumsek, A., Zengin, G., Guler, G. O., Cakmak, Y. S., Duran, A. (2013). Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food and Chemical Toxicology, 55, 290–296. https://doi.org/10.1016/j.fct.2013.01.018

Albayrak, S., Atasagun, B., Aksoy, A. (2017). Comparison of phenolic components and biological activities of two Centaurea spp. obtained by three extraction techniques. Asian Pacific Journal of Tropical Medicine, 10, 599–606. https://doi.org/10.1016/j.apjtm.2017.06.010

Buzhala, M., Mustafa, B., Pulaj, B., Hajdari, A. (2022). Total phenolic, total flavonoid and antioxidant activity of methanolic extracts of some Centaurea species from Kosovo. Macedonian Pharmaceutical Bulletin, 68(2), 65-66. https://doi.org/10.33320/maced.pharm.bull.2022.68.04.027

Carev, I., Ruščić, M., Glumac, M., Politeo, O., Siljak‐Yakovlev, S. (2023). Phytochemical and cytogenetic study of two Centaurea species from Croatia: the particular case of diploid and tetraploid C. salonitana. Chemistry & Biodiversity, 20(5), e202300092. https://doi.org/10.1002/cbdv.202300092

Dai, J., Mumper, R. J. (2010). Plant phenolics extraction, analysis and their antioxidant and anticancer properties. Molecules, 15, 7313–7352. https://doi.org/10.3390/molecules15107313

Foo, S. C., Yusoff, F. M., Ismail, M., Basri, M., Yau, S. K., Khong, N. M. H., Chan, K. W., Ebrahimi, M. (2017). Antioxidant capacities of fucoxanthin-producing algae as influenced by their carotenoid and phenolic contents. Journal of Biotechnology, 241, 175–183. https://doi.org/10.1016/j.jbiotec.2016.11.026

Gharibi, S., Tabatabaei, B. E. S., Saeidi, G., Goli, S. A. H., Talebi, M. (2013). Total phenolic content and antioxidant activity of three Iranian endemic Achillea species. Industrial Crops and Products, 50, 154–158. https://doi.org/10.1016/j.indcrop.2013.07.038

Hernández, I., Alegre, L., Van Breusegem, F., Munné-Bosch, S. (2009). How relevant are flavonoids as antioxidants in plants? Trends in Plant Science, 14, 125–132. https://doi.org/10.1016/j.tplants.2008.12.003

Kenny, O., Smyth, T. J., Walsh, D., Kelleher, C. T., Hewage, C. M., Brunton, N. P. (2014). Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts. Food Chemistry, 161, 79–86. https://doi.org/10.1016/j.foodchem.2014.03.126

Lewis, N. G. (2017). Phenyl propanoids. In: Alscher, R. G. (Ed.), Antioxidants in higher plants (pp. 135–169). Boca Raton: CRC Press.

Nikolić, D., Žabar Popović, A., Vidanović, M., Jenačković Gocić, D., Vasiljević, P., & Jušković, M. (2024). Effect of different extraction solvents on total phenolic and flavonoid contents and antioxidant activity of the Bolboschoenus laticarpus rhizome extracts. Biologica Nyssana, 15(2), 75–81. https://doi.org/10.5281/zenodo.13847305

Piluzza, G., & Bullitta, S. (2011). Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the Mediterranean area. Pharmaceutical Biology, 49(3), 240–247. https://doi.org/10.3109/13880209.2010.501083

Quettier, D. C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M. C., Cayin, J. C., Bailleul, F., & Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 72, 35–42. https://doi.org/10.1016/S0378-8741(00)00196-3

Salachna, P., Pietrak, A., & Łopusiewicz, Ł. (2021). Antioxidant potential of flower extracts from Centaurea spp. depends on their content of phenolics, flavonoids and free amino acids. Molecules, 26(24), 7465. https://doi.org/10.3390/molecules26247465

Salomon, L., Lorenz, P., Bunse, M., Spring, O., Stintzing, F. C., & Kammerer, D. R. (2021). Comparison of the phenolic compound profile and antioxidant potential of Achillea atrata L. and Achillea millefolium L. Molecules, 26(6), 1530. https://doi.org/10.3390/molecules26061530

Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1

Soobrattee, M. A., Neergheen, V. S., Luximon-Ramma, A., Aruoma, O. I., & Bahorun, T. (2005). Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 579(1–2), 200–213. https://doi.org/10.1016/j.mrfmmm.2005.03.023

Stanković, M. S., Radojević, I. D., Stefanović, O. D., Topuzović, M. D., Ćomić, L. R., & Branković, S. R. (2011). Immortelle (Xeranthemum annuum L.) as a natural source of biologically active substances. EXCLI Journal, 10, 230–239. https://doi.org/10.17877/DE290R-3065

Takao, T., Watanabe, N., Yagi, I., & Sakata, K. (1994). A simple screening method for antioxidant and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Bioscience, Biotechnology and Biochemistry, 58, 1780–1783. https://doi.org/10.1271/bbb.58.1780

Tuberoso, C. I. G., Montoro, P., Piacente, S., Corona, G., Deiana, M., Dessì, M. A., Pizza, C., & Cabras, P. (2009). Flavonoid characterization and antioxidant activity of hydroalcoholic extracts from Achillea ligustica All. Journal of Pharmaceutical and Biomedical Analysis, 50(3), 440–448. https://doi.org/10.1016/j.jpba.2009.05.032

Valentão, P., Fernandes, E., Carvalho, F., Andrade, P. B., Seabra, R. M., & Bastos, M. L. (2002). Antioxidative properties of cardoon (Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. Journal of Agricultural and Food Chemistry, 50(17), 4989–4993. https://doi.org/10.1021/jf020225o


Refbacks

  • There are currently no refbacks.